Target
Human ABL1 / c-ABL
Synonyms
ABL1 | Bcr/c-abl oncogene protein | Bcr-Abl | Bcr/abl | C-ABL | ABL | JTK7 | p150 | Tyrosine-protein kinase ABL1 | Proto-oncogene c-Abl | V-abl
Reactivity
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Protein A purified
Immunogen
Synthetic peptide (KLH coupled) derived from the C-Terminus of human c-Abl.
Specificity
Recognizes endogenous levels of Abl proteins. Species cross-reactivity: Human, mouse, rat and drosophila.
Applications
- Immunofluorescence (1:25)
- Western blot (1:1000)
- Immunoprecipitation (1:100)
- ELISA
Usage
Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunofluorescence. Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4?C with gentle shaking, overnight. Recognizes bands at 135kD (c-Abl) (210kD (Bcr-Abl)). Immunoprecipitation: 1:100. Immunofluorescence: 1:25.
Presentation
10 mM HEPES, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 50% Glycerol, No preservatives added
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee