Orders Processing,
Shipping & Receiving,
Warehouse
2 Shaker Rd Suites
B001/B101
Shirley, MA 01464
Production Lab
Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036
1) The 96-well microtiter plate comes pre-bound with specific double-stranded (dsDNA) oligonucleotides. |
2) Following a blocking step samples are added to each well and transcription factor binds to the oligonucleotides. |
3) The wells are washed and primary antibody is added which binds activated transcription factor. |
4) The wells are washed and a HRP-conjugated secondary antibody is added which binds to the primary antibody. |
5) The wells are washed and a solution containing TMB is added. The TMB reacts with the HRP changing from colorless to a blue color. An acid stop solution is then added, the reaction stops, and the blue color changes to yellow. |
1) The 96-well microtiter plate comes pre-bound with specific double-stranded (dsDNA) oligonucleotides. |
2) Following a blocking step samples are added to each well and phosphorylated transcription factor binds to the oligonucleotides. |
3) The wells are washed and a phospho-specific primary antibody is added which binds activated transcription factor. |
4) The wells are washed and a HRP-conjugated secondary antibody is added which binds to the primary antibody. |
5) The wells are washed and a solution containing TMB is added. The TMB reacts with the HRP changing from colorless to a blue color. An acid stop solution is then added, the reaction stops, and the blue color changes to yellow. |