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Catalog Number Size Price
LS-C807721-100 100 µl (1 mg/ml) $379 
LS-C807721-200 200 µl (1 mg/ml) $421 
ACER1 / ASAH3 Antibody - Staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
ACER1 / ASAH3 Antibody - Western blot analysis of extracts of HeLa cells using ASAH3 antibody. Lane 1 was treated with the blocking peptide.
ACER1 / ASAH3 Antibody - Western blot analysis of ASAH3 using LOVO cells whole cells lysates
ACER1 / ASAH3 Antibody - Staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
ACER1 / ASAH3 Antibody - Western blot analysis of extracts of HeLa cells using ASAH3 antibody. Lane 1 was treated with the blocking peptide.
ACER1 / ASAH3 Antibody - Western blot analysis of ASAH3 using LOVO cells whole cells lysates
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Polyclonal Rabbit anti‑Human ACER1 / ASAH3 Antibody (IF, WB) LS‑C807721

Polyclonal Rabbit anti‑Human ACER1 / ASAH3 Antibody (IF, WB) LS‑C807721

Antibody:
ACER1 / ASAH3 Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C807721-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
ACER1 / ASAH3 Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
ASAH3 antibody LS-C807721 is an unconjugated rabbit polyclonal antibody to human ASAH3 (ACER1). Validated for IF, Peptide-ELISA and WB.
Target
Human ACER1 / ASAH3
Synonyms
ACER1 | ALKCDase1 | Acylsphingosine deacylase 3 | Alkaline CDase 1 | AlkCDase 1 | ASAH3 | Alkaline ceramidase 1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide affinity chromatography.
Modifications
Unmodified
Immunogen
Synthesized peptide derived from human ASAH3
Specificity
ASAH3 Antibody detects endogenous levels of total ASAH3.
Applications
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:1000 - 1:3000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ACER1 / ASAH3
Q8TDN7 Q8TDN7

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/3/2024