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Catalog Number Size Price
LS-C750588-20 20 µl $287 
LS-C750588-100 100 µl $431 
ACLY / ATP Citrate Lyase Antibody - Western blot analysis of extracts of NIH/3T3 cells, using Phospho-ACLY-S455 antibody at 1:2000 dilution. NIH/3T3 cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. Blocking buffer: 3% BSA.An ECL Kit was used for detection and the exposure time was 60s.
ACLY / ATP Citrate Lyase Antibody - Immunoprecipitation analysis of 200ug extracts of NIH/3T3 cells, using 3 ug Phospho-ACLY-S455 pAb. Western blot was performed from the immunoprecipitate using Phospho-ACLY-S455 pAb at a dilition of 1:1000. NIH/3T3 cells were treated by Insulin (100 nM) at 37°C for 10 minutes after serum-starvation overnight.
ACLY / ATP Citrate Lyase Antibody - Western blot analysis of extracts of NIH/3T3 cells, using Phospho-ACLY-S455 antibody at 1:2000 dilution. NIH/3T3 cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. Blocking buffer: 3% BSA.An ECL Kit was used for detection and the exposure time was 60s.
ACLY / ATP Citrate Lyase Antibody - Immunoprecipitation analysis of 200ug extracts of NIH/3T3 cells, using 3 ug Phospho-ACLY-S455 pAb. Western blot was performed from the immunoprecipitate using Phospho-ACLY-S455 pAb at a dilition of 1:1000. NIH/3T3 cells were treated by Insulin (100 nM) at 37°C for 10 minutes after serum-starvation overnight.
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Polyclonal Rabbit anti‑Human ACLY / ATP Citrate Lyase Antibody (phospho‑Ser455, WB) LS‑C750588

Polyclonal Rabbit anti‑Human ACLY / ATP Citrate Lyase Antibody (phospho‑Ser455, WB) LS‑C750588

Antibody:
ACLY / ATP Citrate Lyase Rabbit anti-Human Polyclonal (pSer455) Antibody
Application:
WB, IP
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$287
LS-C750588-20
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
ACLY / ATP Citrate Lyase Rabbit anti-Human Polyclonal (pSer455) Antibody
Application:
WB, IP
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
ATP Citrate Lyase antibody LS-C750588 is an unconjugated rabbit polyclonal antibody to ATP Citrate Lyase (ACLY) (pSer455) from human. It is reactive with human and mouse. Validated for IP and WB.
Target
Human ACLY / ATP Citrate Lyase
Synonyms
ACLY | ACL | ATP citrate synthase | ATPCL | ATP citrate lyase | ATP-citrate (pro-S-)-lyase | CLATP | ATP-citrate synthase | Citrate cleavage enzyme | Citrate Lyase
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Epitope
pSer455
Applications
  • Western blot (1:500 - 1:2000)
  • Immunoprecipitation (1:50 - 1:100)
Usage
The predicted MW is 91kDa/119kDa/120kDa, while the observed MW by Western blot was 121kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ACLY / ATP Citrate Lyase
P53396 NM_001096 NP_001087.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/3/2024