Target
Potato ACP1 / Acid Phosphatase
Synonyms
ACP1 | Acid Phosphatase | Acid phosphatase 1, soluble | Acid phosphatase 1 soluble | Adipocyte acid phosphatase | LMW-PTP | HAAP | Red cell acid phosphatase 1 | LMW-PTPase | Protein tyrosine phosphatase
Reactivity
Potato
(tested or 100% immunogen sequence identity)
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Lyophilized
Immunogen
Acid Phosphatase [Potato].
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Acid Phosphatase [Potato]. Cross reactivity against Acid Phosphatase from other sources is unknown.
Applications
- Western blot (1:500 - 1:2000)
- ELISA (1:4000 - 1:20000)
- Dot Blot
Usage
Anti-Acid Phosphatase Biotin Conjugated Antibody has been assayed against 1.0 ug of Acid Phosphatase in a standard capture ELISA using Peroxidase Conjugated Streptavidin ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:8000 to 1:32000 of the reconstitution concentration is suggested for this product. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ACP1 / Acid Phosphatase