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Antibodies > ACTA2 / Smooth Muscle Actin > LS-C800707
 
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Catalog Number Size Price
LS-C800707-100 100 µl (1 mg/ml) $379 
LS-C800707-200 200 µl (1 mg/ml) $421 
ACTA2 / Smooth Muscle Actin Antibody - 1:100 staining rat lung tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
ACTA2 / Smooth Muscle Actin Antibody - 1:100 staining human seminoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
ACTA2 / Smooth Muscle Actin Antibody - 1:100 staining mouse kidney tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
ACTA2 / Smooth Muscle Actin Antibody - 1:100 staining human appendix tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
ACTA2 / Smooth Muscle Actin Antibody - 1:100 staining human skin tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
ACTA2 / Smooth Muscle Actin Antibody - 1:100 staining mouse testis tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
ACTA2 / Smooth Muscle Actin Antibody - The expression of a-SMA and a-Ac-Tub in lung tissue measured by IHC
ACTA2 / Smooth Muscle Actin Antibody - 1:100 staining rat uterine tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
ACTA2 / Smooth Muscle Actin Antibody - 1:100 staining mouse pancreas tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
ACTA2 / Smooth Muscle Actin Antibody - 1:100 staining rat gastric tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
ACTA2 / Smooth Muscle Actin Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
ACTA2 / Smooth Muscle Actin Antibody - Western blot analysis on JK using alpha-SMA antibody. The lane on the left is blocked with the antigen-specific peptide.
ACTA2 / Smooth Muscle Actin Antibody - 1:100 staining rat lung tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
ACTA2 / Smooth Muscle Actin Antibody - 1:100 staining human seminoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
ACTA2 / Smooth Muscle Actin Antibody - 1:100 staining mouse kidney tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
ACTA2 / Smooth Muscle Actin Antibody - 1:100 staining human appendix tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
ACTA2 / Smooth Muscle Actin Antibody - 1:100 staining human skin tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
ACTA2 / Smooth Muscle Actin Antibody - 1:100 staining mouse testis tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
ACTA2 / Smooth Muscle Actin Antibody - The expression of a-SMA and a-Ac-Tub in lung tissue measured by IHC
ACTA2 / Smooth Muscle Actin Antibody - 1:100 staining rat uterine tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
ACTA2 / Smooth Muscle Actin Antibody - 1:100 staining mouse pancreas tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
ACTA2 / Smooth Muscle Actin Antibody - 1:100 staining rat gastric tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
ACTA2 / Smooth Muscle Actin Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
ACTA2 / Smooth Muscle Actin Antibody - Western blot analysis on JK using alpha-SMA antibody. The lane on the left is blocked with the antigen-specific peptide.
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Polyclonal Rabbit anti‑Human ACTA2 / Smooth Muscle Actin Antibody (IHC, IF, WB) LS‑C800707

Polyclonal Rabbit anti‑Human ACTA2 / Smooth Muscle Actin Antibody (IHC, IF, WB) LS‑C800707

Submit Review LSBio Guarantee
Antibody:
ACTA2 / Smooth Muscle Actin Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, IP, Peptide-ELISA
Reactivity:
Human, Mouse, Rat, Bovine
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C800707-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
ACTA2 / Smooth Muscle Actin Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, IP, Peptide-ELISA
Reactivity:
Human, Mouse, Rat, Bovine
Format:
Unconjugated, Unmodified

Specifications

Description
Smooth Muscle Actin antibody LS-C800707 is an unconjugated rabbit polyclonal antibody to Smooth Muscle Actin (ACTA2) from human. It is reactive with human, mouse, rat and other species. Validated for IF, IHC, IP, Peptide-ELISA and WB.
Target
Human ACTA2 / Smooth Muscle Actin
Synonyms
ACTA2 | ACTVS | Alpha-actin-2 | Alpha-cardiac actin | AAT6 | Actin, aortic smooth muscle | ACTSA | Smooth Muscle Actin | MYMY5 | SMA
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide affinity chromatography.
Modifications
Unmodified
Immunogen
Synthetic peptide corresponding to N-Terminus residues of Human alpha smooth muscle Actin.
Specificity
Alpha-SMA antibody detects endogenous levels of alpha-SMA.
Applications
  • IHC (1:50 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
  • Immunoprecipitation
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ACTA2 / Smooth Muscle Actin
P62736 NM_001613 NP_001604.1

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See all ACTA2 / Smooth Muscle Actin Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/22/2024
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6737 Mowry Ave
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United States

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