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Catalog Number	Size	Price
LS-C413493-100	100 µl (1 mg/ml)	$267

ACTB / Beta Actin Antibody - Immunohistochemical analysis of Beta-actin staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

ACTB / Beta Actin Antibody - Immunofluorescent analysis of Beta-actin staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

ACTB / Beta Actin Antibody - Western blot analysis of Beta-actin expression in 293T (A); HeLa (B); mouse kidney (C); rat heart (D); rat brain (E) whole cell lysates.

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Monoclonal Mouse anti‑Human ACTB / Beta Actin Antibody (IHC, IF, WB) LS‑C413493

Name: Anti-ACTB / Beta Actin Antibody | Mouse anti-Human Monoclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C413493-100
Price: 267.0000 USD
Availability: InStock

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Antibody:

ACTB / Beta Actin Mouse anti-Human Monoclonal Antibody

Application:

IHC, IHC-P, ICC, IF, WB

Reactivity:

Human, Monkey, Mouse, Rat, Dog, Rabbit, Chicken

Format:

Unconjugated, Unmodified

$267

LS-C413493-100

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

ACTB / Beta Actin Mouse anti-Human Monoclonal Antibody

Application:

IHC, IHC-P, ICC, IF, WB

Reactivity:

Human, Monkey, Mouse, Rat, Dog, Rabbit, Chicken

Format:

Unconjugated, Unmodified

Specifications

Description

Beta Actin antibody LS-C413493 is an unconjugated mouse monoclonal antibody to Beta Actin (ACTB) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF, IHC and WB.

Target

Human ACTB / Beta Actin

Synonyms

See All ACTB / Beta Actin Antibodies

Host

Mouse

Reactivity

Human, Monkey, Mouse, Rat, Dog, Rabbit, Chicken (tested or 100% immunogen sequence identity)

Clonality

Monoclonal

Conjugations

Unconjugated

Purification

Purified

Modifications

Unmodified

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence of human Beta-actin.

Specificity

Recognizes endogenous levels of Beta-actin protein.

Applications

IHC
IHC - Paraffin (1:100 - 1:200)
ICC (1:100 - 1:200)
Immunofluorescence (1:100 - 1:200)
Western blot (1:2000 - 1:5000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol

Storage

Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About ACTB / Beta Actin

Uniprot: P60709 NCBI: NM_001101 NP_001092.1