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Catalog Number Size Price
LS-C744970-25 25 µl (1 mg/ml) $304 
ADA / Adenosine Deaminase Antibody - Western blot of rabbit Anti-Adenosine Deaminase (Calf Spleen) antibody. Lane 1: Adenosine Deaminase. Lane 2: None. Load: 50 ng per lane. Primary antibody: Adenosine Deaminase antibody at 1:1,000 for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/Observed size: 44 kDa, 44 kDa for Adenosine Deaminase. Other band(s): None.
ADA / Adenosine Deaminase Antibody - Biotin conjugated anti adenosine deaminase was used to detect adenosine deaminase in mouse pancreas lysate (Left, Lane 1, 30 ul) under reducing conditions. The antibody was also used to detect purified Adenosine Deaminase (right, Lane 2), and endogenous Adenosine Deaminase in whole cell lysate from Jurkat and Raji cells (1:1 mixture, lane 3,) as well as Mouse Pancreas and Liver (1:1 mixture, lane 4). Lysates were run on 4-20% gel 140V under reducing conditions, transferred for 30 minutes at 100 V and blocked with 3% Fish Gel (left) or 3% BSA.
ADA / Adenosine Deaminase Antibody - Western blot of rabbit Anti-Adenosine Deaminase (Calf Spleen) antibody. Lane 1: Adenosine Deaminase. Lane 2: None. Load: 50 ng per lane. Primary antibody: Adenosine Deaminase antibody at 1:1,000 for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/Observed size: 44 kDa, 44 kDa for Adenosine Deaminase. Other band(s): None.
ADA / Adenosine Deaminase Antibody - Biotin conjugated anti adenosine deaminase was used to detect adenosine deaminase in mouse pancreas lysate (Left, Lane 1, 30 ul) under reducing conditions. The antibody was also used to detect purified Adenosine Deaminase (right, Lane 2), and endogenous Adenosine Deaminase in whole cell lysate from Jurkat and Raji cells (1:1 mixture, lane 3,) as well as Mouse Pancreas and Liver (1:1 mixture, lane 4). Lysates were run on 4-20% gel 140V under reducing conditions, transferred for 30 minutes at 100 V and blocked with 3% Fish Gel (left) or 3% BSA.
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Polyclonal Rabbit anti‑Bovine ADA / Adenosine Deaminase Antibody (Liquid, WB) LS‑C744970

Polyclonal Rabbit anti‑Bovine ADA / Adenosine Deaminase Antibody (Liquid, WB) LS‑C744970

Antibody:
ADA / Adenosine Deaminase Rabbit anti-Bovine Polyclonal (Liquid) Antibody
Application:
WB, IP, ELISA
Reactivity:
Bovine
Format:
Unconjugated, Liquid
Price
Catalog Number
$304
LS-C744970-25
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
ADA / Adenosine Deaminase Rabbit anti-Bovine Polyclonal (Liquid) Antibody
Application:
WB, IP, ELISA
Reactivity:
Bovine
Format:
Unconjugated, Liquid

Specifications

Description
Adenosine Deaminase antibody LS-C744970 is an unconjugated rabbit polyclonal antibody to bovine Adenosine Deaminase (ADA). Validated for ELISA, IP and WB.
Target
Bovine ADA / Adenosine Deaminase
Synonyms
ADA | Adenosine deaminase | ADA1 | Adenosine aminohydrolase
Host
Rabbit
Reactivity
Bovine (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Liquid
Immunogen
Adenosine Deaminase [Calf Spleen].
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Adenosine Deaminase [Calf Spleen]. Cross reactivity against Adenosine Deaminase from other tissues and species may occur but have not been specifically determined.
Applications
  • Western blot (1:500 - 1:5000)
  • Immunoprecipitation (1:100)
  • ELISA (1:5000 - 1:20000)
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ADA / Adenosine Deaminase
P00813 NM_000022 NP_000013.2

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Requested From: United States
Date Requested: 11/3/2024