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Antibodies > ADD1 / Adducin Alpha > LS-C353875
 
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Catalog Number Size Price
LS-C353875-100 100 µl (1 mg/ml) $299 
ADD1 / Adducin Alpha Antibody - Immunohistochemical analysis of Alpha-adducin staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
ADD1 / Adducin Alpha Antibody - Immunofluorescent analysis of Alpha-adducin staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
ADD1 / Adducin Alpha Antibody - Western blot analysis of Alpha-adducin expression in HeLa (A); Raw264.7 (B); rat brain (C) whole cell lysates.
ADD1 / Adducin Alpha Antibody - Immunohistochemical analysis of Alpha-adducin staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
ADD1 / Adducin Alpha Antibody - Immunofluorescent analysis of Alpha-adducin staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
ADD1 / Adducin Alpha Antibody - Western blot analysis of Alpha-adducin expression in HeLa (A); Raw264.7 (B); rat brain (C) whole cell lysates.
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Polyclonal Rabbit anti‑Human ADD1 / Adducin Alpha Antibody (Internal, IHC, IF, WB) LS‑C353875

Polyclonal Rabbit anti‑Human ADD1 / Adducin Alpha Antibody (Internal, IHC, IF, WB) LS‑C353875

Submit Review LSBio Guarantee
Antibody:
ADD1 / Adducin Alpha Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat, Pig
Format:
Unconjugated, Unmodified
Price
Catalog Number
$299
LS-C353875-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
ADD1 / Adducin Alpha Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat, Pig
Format:
Unconjugated, Unmodified

Specifications

Description
Adducin Alpha antibody LS-C353875 is an unconjugated rabbit polyclonal antibody to Adducin Alpha (ADD1) (Internal) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF, IHC and WB.
Target
Human ADD1 / Adducin Alpha
Synonyms
ADD1 | ADDA | Adducin 1 (alpha) | Alpha-adducin
Host
Rabbit
Reactivity
Human, Mouse, Rat, Pig (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the central region of human Alpha-adducin.
Epitope
Internal
Specificity
Recognizes endogenous levels of Alpha-adducin protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ADD1 / Adducin Alpha
P35611 NM_014189 NP_054908.2

Publications (0)

Submit a Publication for LS-C353875

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Featured Products

ADD1 / Adducin Alpha Antibody - Anti-ADD1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
IHC‑plus™ ADD1 / Adducin Alpha Antibody (C‑Terminus, clone EP734Y) LS‑B5957
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, ICC, Western blot
ADD1 / Adducin Alpha Antibody - Anti-ADD1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
IHC‑plus™ ADD1 / Adducin Alpha Antibody (aa411‑460) LS‑B7561
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Peptide Enzyme-Linked Immunosorbent Assay
ADD1 / Adducin Alpha Antibody - Immunohistochemical analysis of Alpha-adducin (pT445) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP-conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
ADD1 / Adducin Alpha Antibody (phospho‑Thr445) LS‑C358407
Species: Human, Mouse, Rat, Pig
Applications: IHC, IHC - Paraffin, Western blot
ADD1 / Adducin Alpha Antibody - Immunohistochemistry of paraffin-embedded Human lung cancer using ADD1 Polyclonal Antibody at dilution of 1:50.
ADD1 / Adducin Alpha Antibody LS‑C403712
Species: Human, Mouse, Rat
Applications: IHC, ELISA
EPO / Erythropoietin Protein - Recombinant Erythropoietin By SDS-PAGE
Human EPO / Erythropoietin Protein (Recombinant His-GST) (aa28-193) - LS-G11249
Source: E. coli
Tag: His-GST
See all ADD1 / Adducin Alpha Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/3/2024
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