Target
Mouse Adiponectin
Synonyms
ADIPOQ | Adiponectin | ADPN | ACDC | ADIPQTL1 | APM-1 | APM1 | ACRP30 | Gelatin-binding protein 28 | GBP28 | Gelatin-binding protein
Reactivity
Mouse
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k
Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified from tissue culture supernatant
Immunogen
Recombinant mouse adiponectin.
Specificity
Recognizes mouse adiponectin. Detects a band of ~30kDa by Western blot. Weakly cross-reacts with human and rat adiponectin.
Applications
- Western blot (1:5000 - 1:10000)
- Immunoprecipitation
- ELISA (1:5000 - 1:10000)
Usage
ELISA - Direct and indirect: 1:5000-1:10000. Immunoprecipitation - 1-5ul per sample in a final reaction volume of 500-1000ul. Western blot - 1:5000-1:10000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature.
Presentation
PBS, pH 7.4, no preservatives.
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee