Target
Human AKT1 + AKT2 + AKT3
Reactivity
Human, Monkey, Mouse, Rat
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k
Monoclonal
Immunogen
Synthetic peptide corresponding to residues in the C-Terminus sequence of human Akt protein.
Specificity
Recognizes endogenous levels of total Akt protein. Does not cross-react with other related proteins. Species cross-reactivity: Mouse, rat and monkey.
Applications
- Western blot (1:1000)
- Immunoprecipitation (1:50)
- Flow Cytometry (1:50)
Usage
Suitable for use in Flow Cytometry, Western Blot and Immunoprecipitation. Flow Cytometry: 1:50. Western Blot: 1:1000. Immunoprecipitation: 1:50. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
136 mM sodium chloride, 2.6 mM KCl, 12 mM sodium phosphate dibasic, pH 7.4, 2 mg/ml BSA, 50% glycerol
Storage
May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for up to 1 year.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee