Human AKT1

Mouse

Human, Chimpanzee, Mouse, Rat (tested or 100% immunogen sequence identity)

Chimpanzee, Rat (at least 90% immunogen sequence identity)

IgG Monoclonal

14E5.16C8.25F6

Unconjugated

Protein A affinity chromatography

Unmodified

Anti-AKT Antibody was produced by repeated immunizations in mice with a synthetic peptide corresponding to residues internal to the human AKT1 protein.

Internal

This antibody is specific for human and mouse AKT protein. A BLAST analysis was used to suggest cross-reactivity with AKT1, AKT2, and AKT3 from human, mouse, rat and chimpanzee sources based on 100% homology with the immunizing sequence. Cross-reactivity with AKT1, 2, 3 was determined with Western Blot. Cross reactivity of AKT from other sources has not been determined.

• IHC (20 µg/ml)
• Western blot (1:500 - 1:3000)
• Flow Cytometry
• ELISA (1:20000)

Anti-AKT Antibody is suitable for ELISA, immunohistochemistry, immunoprecipitation and western blotting. Expect a band approximately 56 kD in size corresponding to AKT protein by western blotting in the appropriate cell lysate or extract. This monoclonal antibody reacts with human and mouse AKT. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry we recommend the use of fresh frozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative.

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P31749 NCBI: NM_005163 NP_005154.2