Products
Research Areas
COVID-19
Resources
Login
Quick Order
Cart Cart lightblue
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Contact Us

Locations


Orders Processing,
Shipping & Receiving,
Warehouse

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464


Production Lab

Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036

Telephone Numbers



Tel: +1 (800) 227-6666


Contact Us



Additional Contact Details

Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-C750601-20 20 µl $287 
LS-C750601-100 100 µl $431 
AKT1S1 / PRAS40 Antibody - Western blot analysis of extracts of HeLa cells, using Phospho-AKT1S1-T246 antibody at 1:2000 dilution. HeLa cells were treated by Insulin (0.01U/ml) for 15 minutes after serum-starvation overnight. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. Blocking buffer: 3% BSA.An ECL Kit was used for detection and the exposure time was 90s.

Polyclonal Rabbit anti‑Human AKT1S1 / PRAS40 Antibody (phospho‑Thr246, WB) LS‑C750601

Polyclonal Rabbit anti‑Human AKT1S1 / PRAS40 Antibody (phospho‑Thr246, WB) LS‑C750601

Antibody:
AKT1S1 / PRAS40 Rabbit anti-Human Polyclonal (pThr246) Antibody
Application:
WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$287
LS-C750601-20
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
AKT1S1 / PRAS40 Rabbit anti-Human Polyclonal (pThr246) Antibody
Application:
WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
PRAS40 antibody LS-C750601 is an unconjugated rabbit polyclonal antibody to PRAS40 (AKT1S1) (pThr246) from human. It is reactive with human and mouse. Validated for WB.
Target
Human AKT1S1 / PRAS40
Synonyms
AKT1S1 | PRAS40 | Proline-rich AKT1 substrate 1 | Lobe
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Epitope
pThr246
Applications
  • Western blot (1:500 - 1:2000)
Usage
The predicted MW is 13kDa/27kDa/29kDa, while the observed MW by Western blot was 45kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About AKT1S1 / PRAS40
Q96B36 NM_032375 NP_115751.3

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/3/2024