Human AKT2

Rabbit

Human (tested or 100% immunogen sequence identity)

Rat (at least 90% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Affinity purified

Unmodified

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to C-Terminal region near aa 450-475 (of 481) of human AKT2 conjugated to Keyhole Limpet Hemocyanin (KLH).

aa450-475

The antibody detects both unphosphorylated and phosphorylated forms of the protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Cross reactivity with AKT2 from other species has not been determined, however, the sequence of the immunogen shows 85% identity to mouse and 92% identity with rat, therefore, cross reactivity is expected.

• Western blot (1:1000)
• Flow Cytometry
• ELISA (1:4000 - 1:16000)

This antibody was tested by ELISA and immunoblotting and was found to be reactive with both unphosphorylated and phosphorylated AKT2 in a lysate of HEK293 cells. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. This product has been assayed by immunoblot against a HEK293 cell lysate and is reactive at a 1:1000 dilution showing a band at approximately 60 kD. A working dilution of 1:4000 to 1:16000 is suggested for this product in a standard capture ELISA using TMB as a substrate for 30 minutes at room temperature against 0.1 ug of the immunizing peptide. Researchers should determine optimal titers for other applications.

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Store vial at -20°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P31751 NCBI: NM_001626 NP_001617.1