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Antibodies > AKT2 > LS-C351826
 
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Catalog Number Size Price
LS-C351826-100 100 µl (1 mg/ml) $299 
AKT2 Antibody - Immunohistochemical analysis of AKT2 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
AKT2 Antibody - Immunofluorescent analysis of AKT2 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
AKT2 Antibody - Western blot analysis of AKT2 expression in HeLa (A); HepG2 (B); NIH3T3 (C); mouse brain (D); rat brain (E) whole cell lysates.
AKT2 Antibody - Immunohistochemical analysis of AKT2 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
AKT2 Antibody - Immunofluorescent analysis of AKT2 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
AKT2 Antibody - Western blot analysis of AKT2 expression in HeLa (A); HepG2 (B); NIH3T3 (C); mouse brain (D); rat brain (E) whole cell lysates.
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Polyclonal Rabbit anti‑Human AKT2 Antibody (C‑Terminus, IHC, IF, WB) LS‑C351826

Polyclonal Rabbit anti‑Human AKT2 Antibody (C‑Terminus, IHC, IF, WB) LS‑C351826

Submit Review LSBio Guarantee
Antibody:
AKT2 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat, Dog, Chicken
Format:
Unconjugated, Unmodified
Price
Catalog Number
$299
LS-C351826-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
AKT2 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat, Dog, Chicken
Format:
Unconjugated, Unmodified

Specifications

Description
AKT2 antibody LS-C351826 is an unconjugated rabbit polyclonal antibody to AKT2 (C-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF, IHC and WB.
Target
Human AKT2
Synonyms
AKT2 | PKB beta | PKBBETA | PRKBB | Protein kinase Akt-2 | Protein kinase B beta | RAC-PK-beta | Rac protein kinase beta | RAC-BETA | HIHGHH | PKBB
Host
Rabbit
Reactivity
Human, Mouse, Rat, Dog, Chicken (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-Terminus of human AKT2.
Epitope
C-Terminus
Specificity
Recognizes endogenous levels of AKT2 protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About AKT2
P31751 NM_001626 NP_001617.1

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Featured Products

Product
AKT2 Antibody (phospho‑Ser474, PE) LS‑C218598
Species: Human
Applications: ELISA, Dot Blot
AKT2 Antibody - IHC of paraffin-embedded liver tissue using anti-AKT2 mouse monoclonal antibody. (Dilution 1:50).
AKT2 Antibody (clone OTI4H7) LS‑C114889
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Product
AKT2 Antibody (aa214‑451, clone 148CT5.4.1.4, PE) LS‑C221216
Species: Human, Mouse
Applications: IHC, ICC, Immunofluorescence, Western blot, ELISA
AKT2 Antibody - AKT2 Monoclonal Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human prostate carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.
AKT2 Antibody (aa214‑451) LS‑C156232
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
AKT2 Antibody - Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with AKT2 Antibody (S474), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
AKT2 Antibody (Ser474) LS‑C98942
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
AKT2 Antibody - Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with AKT2 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
AKT2 Antibody (aa416‑444) LS‑C98940
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
See all AKT2 Antibodies

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Requested From: United States
Date Requested: 11/25/2024
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