Human AKT3

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

25F6.F6.D8

Unconjugated

Protein A affinity chromatography

Unmodified

Anti-AKT3 Antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT3 protein.

Internal

The antibody detects both unphosphorylated and phosphorylated forms of the protein. Anti-AKT3 antibody was purified from ascites by Protein A chromatography. Cross reactivity with AKT3 from other species has not been determined, however, the sequence of the immunogen shows 100% identity to human, mouse, and rat, therefore, cross reactivity is expected. Cross-reactivity with AKT2 and AKT has not been determined.

• IHC (20 µg/ml)
• Western blot (1:500 - 1:2000)
• Flow Cytometry
• ELISA (1:2000 - 1:10000)

Anti-AKT3 Antibody is suitable for western blotting to detect a single band of the expected apparent molecular weight and for ELISA. Researchers should determine optimal titers for applications that are not stated below.

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Store at -20°C. Aliquot to avoid freeze-thaw cycles. Store undiluted.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q9Y243 NCBI: NM_005465 NP_005456.1