Bovine ALB / Serum Albumin

Rabbit

Bovine (tested or 100% immunogen sequence identity)

Polyclonal

Horseradish Peroxidase. Also available Unconjugated or conjugated with FITC, Biotin.

Purified IgG

Unmodified

Purified albumin isolated from pooled bovine serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Bovine albumin.

• IHC
• IHC - Paraffin
• ICC
• ELISA
• Dot Blot
• Applications tested for the base form of this product only

Tested in immunoelectrophoresis and double radial immunodiffusion against pooled normal bovine serum and purified bovine albumin. One characteristic precipitin line is obtained against pooled normal bovine serum using different antigen/antibody concentration ratio's. Precipitin lines against normal bovine serum and purified bovine albumin give a reaction of full identity. In enzyme-immunocytochemical and immunohistochemical staining for the detection of albumin, of appropriately treated cell and tissue substrates at the cellular and subcellular level. In non-isotopic assay methodology (e. g. ELISA) to identify and measure albumin in bovine serum or other body fluid. In electron microscopy, since the complex between the conjugated antibody and the antigen also has electron-dense properties. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:500. in ELISA and comparable non-precipitating antibody-binding assays between 1:2000 and 1:10000. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Lyophilized from PBS, pH 7.2, No preservatives added

1 ml Distilled water

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P02768 NCBI: NM_000477 NP_000468.1