Human ALCAM / CD166

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

Biotin

Immunoaffinity purified

Unmodified

Purified, NS0-derived, recombinant human ALCAM extracellular domain (rhALCAM; aa 28-527; Accession No. Q13740).

aa28-527

Detects Recombinant, Human ALCAM in Western blots. No cross-reactivity seen with Recombinant, Murine ALCAM, rhBCAM, rhTROP-2, rhEpCAM, rhMCAM, rhNCAML1, rmMadCAM or rmOCAM.

• Western blot (1 - 2 µg/ml)
• Flow Cytometry (25 µg/ml)

Suitable for use in Western Blot, Flow Cytometry. Western Blot: 1-2 ug/ml. Using a colorimetric detection system, the detection limit for rhALCAM is approximately 5 ng/lane under non-reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Flow cytometry: 25 ug/ml and add 10 ul of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 ul of a 25 ug/ml stock solution of a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Sterile-filtered, lyophilized powder in PBS, pH 7.2, BSA

Reconstitute in sterile PBS.

Lyophilized powder may be stored at 4°C for short-term only. Reconstituted product is stable for 1 year at -20°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q13740 NCBI: NM_001627 NP_001618.2