Target
Rabbit ALDOA / Aldolase A
Synonyms
ALDOA | ALDA | Aldolase | GSD12 | Muscle-type aldolase | Lung cancer antigen NY-LU-1
Reactivity
Rabbit, Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Delipidation, salt fractionation and ion exchange chromatography
Modifications
Lyophilized
Immunogen
Aldolase [Rabbit Muscle].
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, purified and partially purified Aldolase [Rabbit Muscle].
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Western blot (1:1000 - 1:5000)
- Immunoprecipitation (1:100)
- ELISA (1:7000)
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ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Usage
Immunohistochemistry: LS-B1461 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1461 was determined to be 5 ug/ml.
Presentation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee