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Catalog Number Size Price
LS-C209623-100 100 µg (1 mg/ml) $552 
ALDOA / Aldolase A Antibody - IgG purified antibody to rabbit muscle aldolase was used at a 1:1000 dilution to detect human aldolase by western blot. A whole cell lysate prepared from human derived A293 cells was loaded on a 4-12% Tris glycine gradient gel for SDS-PAGE. The gel was transferred to nitro-cellulose using standard techniques. Antibody reaction with the membrane occurred overnight at 4°C in TTBS supplemented with 2% non-fat dry milk. Color was allowed to develop using SuperSignal West Pico Chemiluminescent Substrate (PIERCE). Other detection methods will yield similar results. This antibody clearly detects a band at ~41 kDa consistent with human aldolase. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Polyclonal Goat anti‑Rabbit ALDOA / Aldolase A Antibody (Lyophilized, HRP, WB) LS‑C209623

Polyclonal Goat anti‑Rabbit ALDOA / Aldolase A Antibody (Lyophilized, HRP, WB) LS‑C209623

Antibody:
ALDOA / Aldolase A Goat anti-Rabbit Polyclonal (Lyophilized) (HRP) Antibody
Application:
WB, IP, ELISA
Reactivity:
Rabbit, Human
Format:
Horseradish Peroxidase, Lyophilized
Price
Catalog Number
$552
LS-C209623-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
ALDOA / Aldolase A Goat anti-Rabbit Polyclonal (Lyophilized) (HRP) Antibody
Application:
WB, IP, ELISA
Reactivity:
Rabbit, Human
Format:
Horseradish Peroxidase, Lyophilized

Specifications

Description
Aldolase A antibody LS-C209623 is an HRP-conjugated goat polyclonal antibody to Aldolase A (ALDOA) from rabbit. It is reactive with human and rabbit. Validated for ELISA, IP and WB.
Target
Rabbit ALDOA / Aldolase A
Synonyms
ALDOA | ALDA | Aldolase | GSD12 | Muscle-type aldolase | Lung cancer antigen NY-LU-1
Host
Goat
Reactivity
Rabbit, Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Horseradish Peroxidase
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Lyophilized
Immunogen
Aldolase [Rabbit Muscle].
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Aldolase [Rabbit Muscle].
Applications
  • Western blot (1:500 - 1:5000)
  • Immunoprecipitation (1:100)
  • ELISA (1:1000 - 1:5000)
Usage
Anti-Aldolase has been assayed against 1.0 ug of Aldolase [Rabbit Muscle] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:1000 to 1:2000 of the reconstitution concentration is suggested for this product. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ALDOA / Aldolase A
P04075 NM_000034 NP_000025.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/3/2024