Target
Rabbit ALDOA / Aldolase A
Synonyms
ALDOA | ALDA | Aldolase | GSD12 | Muscle-type aldolase | Lung cancer antigen NY-LU-1
Reactivity
Rabbit, Human
(tested or 100% immunogen sequence identity)
Conjugations
Horseradish Peroxidase
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Lyophilized
Immunogen
Aldolase [Rabbit Muscle].
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Aldolase [Rabbit Muscle].
Applications
- Western blot (1:500 - 1:5000)
- Immunoprecipitation (1:100)
- ELISA (1:1000 - 1:5000)
Usage
Anti-Aldolase has been assayed against 1.0 ug of Aldolase [Rabbit Muscle] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:1000 to 1:2000 of the reconstitution concentration is suggested for this product. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee