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Catalog Number Size Price
LS-C744593-25 25 µl (80 mg/ml) $304 
ANGPT1 / Angiopoietin-1 Antibody - Anti-ANG1 antibody was diluted 1:500 to detect ANG1 in human lung tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
ANGPT1 / Angiopoietin-1 Antibody - Rabbit anti-ANG1 was used at a 1:500 dilution to detect mouse Ang-1 by western blot against supernatants of mouse angiopoietin-expressing endothelial cells. Lane 1 - wt endothelial cells. Lane 2 - mouse Ang-1 (clone 1-8) expressing cells. Lane 3 - mouse Ang-1 (clone 1-15) expressing cells. Lane 4 - mouse Ang-2 (clone 2-9) expressing cells. Approximately 20 µg of each lysate was used for 10% SDS-PAGE. Immunoprecipitation preceded the reaction with primary antibody at room temperature for 1 h. After subsequent washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG preceded color development.
ANGPT1 / Angiopoietin-1 Antibody - Anti-ANG1 antibody was diluted 1:500 to detect ANG1 in human lung tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
ANGPT1 / Angiopoietin-1 Antibody - Rabbit anti-ANG1 was used at a 1:500 dilution to detect mouse Ang-1 by western blot against supernatants of mouse angiopoietin-expressing endothelial cells. Lane 1 - wt endothelial cells. Lane 2 - mouse Ang-1 (clone 1-8) expressing cells. Lane 3 - mouse Ang-1 (clone 1-15) expressing cells. Lane 4 - mouse Ang-2 (clone 2-9) expressing cells. Approximately 20 µg of each lysate was used for 10% SDS-PAGE. Immunoprecipitation preceded the reaction with primary antibody at room temperature for 1 h. After subsequent washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG preceded color development.
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Polyclonal Rabbit anti‑Mouse ANGPT1 / Angiopoietin‑1 Antibody (aa15‑45, IHC, WB) LS‑C744593

Polyclonal Rabbit anti‑Mouse ANGPT1 / Angiopoietin‑1 Antibody (aa15‑45, IHC, WB) LS‑C744593

Antibody:
ANGPT1 / Angiopoietin-1 Rabbit anti-Mouse Polyclonal (aa15-45) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Mouse, Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$304
LS-C744593-25
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
ANGPT1 / Angiopoietin-1 Rabbit anti-Mouse Polyclonal (aa15-45) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Mouse, Human
Format:
Unconjugated, Unmodified

Specifications

Description
Angiopoietin-1 antibody LS-C744593 is an unconjugated rabbit polyclonal antibody to Angiopoietin-1 (ANGPT1) (aa15-45) from mouse. It is reactive with human and mouse. Validated for ELISA, IHC and WB.
Target
Mouse ANGPT1 / Angiopoietin-1
Synonyms
ANGPT1 | Angiopoietin 1 | Angiopoietin-1 | AGPT | AGP1 | ANG-1 | ANG1 | KIAA0003
Host
Rabbit
Reactivity
Mouse, Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Delipidated and defibrinated
Modifications
Unmodified
Immunogen
This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to a N-terminus region near aa 15-45 of mouse angiopoietin 1 protein conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling.
Epitope
aa15-45
Specificity
This reagent cross-reacts with human angiopoietin 1. Partially cross reactivity is noted with human angiopoietin 2. This product was prepared from monospecific antiserum by a delipidation and defibrination.
Applications
  • IHC (1:200 - 1:800)
  • Western blot (1:500 - 1:2000)
  • ELISA (1:5000 - 1:25000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ANGPT1 / Angiopoietin-1
Q15389 NM_001146 NP_001137.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/22/2024