Human ANO1 / DOG1 / TMEM16A

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

DG1/447 + DOG-1.1

Unconjugated

Protein G purified

Unmodified. Also available Ready-to-Use, Azide-free, BSA-free.

Recombinant human DOG1 protein (DG1/447) and amino acids 904-986 (DOG-1.1) were used as the immunogens for this DOG-1 antibody cocktail.

aa904-986

Human ANO1 / DOG1 / TMEM16A

• IHC - Paraffin (0.25 - 0.5 µg/ml)

Expression of DOG-1 protein is elevated in the gastrointestinal stromal tumors (GISTs), c-kit signaling-driven mesenchymal tumors of the GI tract. DOG-1 is rarely expressed in other soft tissue tumors, which, due to appearance, may be difficult to diagnose. Immunoreactivity for DOG-1 has been reported in 97.8 percent of scorable GISTs, including all c-kit negative GISTs. Overexpression of DOG-1 has been suggested to aid in the identification of GISTs, including Platelet-Derived Growth Factor Receptor Alpha mutants that fail to express c-kit antigen. The overall sensitivity of DOG1 and c-kit in GISTs is nearly identical: 94.4% vs. 94.7%. The optimal dilution of the DOG-1 antibody for each application should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

1X PBS, 0.1 mg/ml BSA, 0.05% sodium azide

Store at 2°C to 8°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q5XXA6 NCBI: NM_018043 NP_060513.5