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Catalog Number Size Price
LS-C756420-10 10 µg $318 
LS-C756420-100 100 µg $470 
ANO1 / DOG1 / TMEM16A Antibody - Western blot analysis of TMEM16A using anti-TMEM16A antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysate,Lane 2: human HepG2 whole cell lysate,Lane 3: human A549 whole cell lysate,Lane 4: human PANC-1 whole cell lysate,Lane 5: human SK-OV-3 whole cell lysate,Lane 6: human SGC-7901 whole cell lysate,Lane 7: human COLO-320 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM16A antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TMEM16A at approximately 130KD. The expected band size for TMEM16A is at 114KD.

Polyclonal Rabbit anti‑Human ANO1 / DOG1 / TMEM16A Antibody (WB) LS‑C756420

Polyclonal Rabbit anti‑Human ANO1 / DOG1 / TMEM16A Antibody (WB) LS‑C756420

Antibody:
ANO1 / DOG1 / TMEM16A Rabbit anti-Human Polyclonal Antibody
Application:
WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C756420-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
ANO1 / DOG1 / TMEM16A Rabbit anti-Human Polyclonal Antibody
Application:
WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
TMEM16A antibody LS-C756420 is an unconjugated rabbit polyclonal antibody to TMEM16A (ANO1 / DOG1) from human. It is reactive with human, mouse and rat. Validated for WB.
Target
Human ANO1 / DOG1 / TMEM16A
Synonyms
ANO1 | Oral cancer overexpressed 2 | Transmembrane protein 16A | ORAOV2 | Anoctamin-1 | DOG1 | TAOS2 | TMEM16A
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence of human TMEM16A (QQIHKEK VLMVELFMREEQDKQQLLETWMEKERQKDE).
Specificity
No cross reactivity with other proteins.
Applications
  • Western blot (0.1 - 0.5 µg/ml)
Usage
Applications should be user optimized.
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
After reconstitution, may be stored at 4°C for 1 month. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ANO1 / DOG1 / TMEM16A
Q5XXA6 NM_018043 NP_060513.5

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 7/22/2024