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Antibodies > APEX1 / APE1 > LS-C756627
 
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Catalog Number Size Price
LS-C756627-10 10 µg $318 
LS-C756627-100 100 µg $470 
APEX1 / APE1 Antibody - IHC analysis of APE1 using anti-APE1 antibody. APE1 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml mouse anti-APE1 Antibody overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
APEX1 / APE1 Antibody - Western blot analysis of APE1 using anti-APE1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human COLO-320 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: Rabbit IgG, Lane 6: Marker 1113, Lane 7: human A549 whole cell lysates, Lane 8: human PANC-1 whole cell lysates, Lane 9: human 22RV1 whole cell lysates, Lane 10: human MDA-MB-453 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-APE1 antigen affinity purified monoclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a Biotin Conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system.
APEX1 / APE1 Antibody - IHC analysis of APE1 using anti-APE1 antibody. APE1 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml mouse anti-APE1 Antibody overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
APEX1 / APE1 Antibody - Western blot analysis of APE1 using anti-APE1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human COLO-320 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: Rabbit IgG, Lane 6: Marker 1113, Lane 7: human A549 whole cell lysates, Lane 8: human PANC-1 whole cell lysates, Lane 9: human 22RV1 whole cell lysates, Lane 10: human MDA-MB-453 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-APE1 antigen affinity purified monoclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a Biotin Conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system.
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Monoclonal Mouse anti‑Human APEX1 / APE1 Antibody (IHC, WB) LS‑C756627

Monoclonal Mouse anti‑Human APEX1 / APE1 Antibody (IHC, WB) LS‑C756627

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Antibody:
APEX1 / APE1 Mouse anti-Human Monoclonal Antibody
Application:
IHC-P, IHC-Fr, ICC, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C756627-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
APEX1 / APE1 Mouse anti-Human Monoclonal Antibody
Application:
IHC-P, IHC-Fr, ICC, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
APE1 antibody LS-C756627 is an unconjugated mouse monoclonal antibody to human APE1 (APEX1). Validated for Flow, ICC, IHC and WB.
Target
Human APEX1 / APE1
Synonyms
APEX1 | AP endonuclease class I | AP lyase | APE | APE-1 | APEN | APE1 | APEX nuclease | Redox factor-1 | AP endonuclease 1 | APEX | APX | Protein REF-1 | REF-1 | REF1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2b Monoclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E.coli-derived human APE1 recombinant protein (Position: P2-L318). Human APE1 shares 94% and 93% amino acid (aa) sequence identity with mouse and rat APE1, respectively.
Specificity
No cross reactivity with other proteins.
Applications
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • IHC - Frozen (0.5 - 1 µg/ml)
  • ICC (0.5 - 1 µg/ml)
  • Western blot (0.1 - 0.5 µg/ml)
  • Flow Cytometry (1 - 3 µg/10E6 cells)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
After reconstitution, may be stored at 4°C for 1 month. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About APEX1 / APE1
P27695 NM_001641 NP_001632.2

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Species: Human, Mouse, Rat
Applications: IHC, IHC - Frozen, ICC, Western blot, Immunoprecipitation
APEX1 / APE1 Antibody - Anti-APEX1 / APE1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC‑plus™ APEX1 / APE1 Antibody (aa2‑15) LS‑B3924
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
APEX1 / APE1 Antibody
APEX1 / APE1 Antibody LS‑C136888
Species: Human
Applications: Western blot, ELISA
APEX1 / APE1 Antibody - APEX1 / APE1 antibody Western blot of 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
APEX1 / APE1 Antibody (aa215‑264) LS‑C116767
Species: Human
Applications: Western blot
APEX1 / APE1 Antibody - APEX1 / APE1 antibody ARP32651_T100-NP_542379-APEX1 (APEX nuclease (multifunctional DNA repair enzyme) 1) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human lung. This image was taken for the unconjugated form of this product. Other forms have not been tested.
APEX1 / APE1 Antibody (aa79‑128) LS‑C30761
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Product
APEX1 / APE1 Antibody (aa219‑318) LS‑C306289
Species: Human
Applications: ELISA
See all APEX1 / APE1 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 5/15/2024
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