Products
Research Areas
COVID-19
Resources
Login
Quick Order
Cart Cart lightblue
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Contact Us

Locations


Orders Processing,
Shipping & Receiving,
Warehouse

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464


Production Lab

Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036

Telephone Numbers



Tel: +1 (800) 227-6666


Contact Us



Additional Contact Details

Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-C746818-20 20 µl $287 
LS-C746818-100 100 µl $431 
APEX1 / APE1 Antibody - Immunofluorescence analysis of A549 cells using APEX1 antibody.
APEX1 / APE1 Antibody - Immunofluorescence analysis of HeLa cells using APEX1 antibody. Blue: DAPI for nuclear staining.
APEX1 / APE1 Antibody - Western blot analysis of extracts from normal (control) and APEX1 knockout (KO) 293T cells, using APEX1 antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 1s.
APEX1 / APE1 Antibody - Western blot analysis of extracts of various cell lines, using APEX1 antibody. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
APEX1 / APE1 Antibody - Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug APEX1 antibody. Western blot was performed from the immunoprecipitate using APEX1 antibody at a dilition of 1:1000.
APEX1 / APE1 Antibody - Immunofluorescence analysis of A549 cells using APEX1 antibody.
APEX1 / APE1 Antibody - Immunofluorescence analysis of HeLa cells using APEX1 antibody. Blue: DAPI for nuclear staining.
APEX1 / APE1 Antibody - Western blot analysis of extracts from normal (control) and APEX1 knockout (KO) 293T cells, using APEX1 antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 1s.
APEX1 / APE1 Antibody - Western blot analysis of extracts of various cell lines, using APEX1 antibody. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
APEX1 / APE1 Antibody - Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug APEX1 antibody. Western blot was performed from the immunoprecipitate using APEX1 antibody at a dilition of 1:1000.
1 of 5
2 of 5
3 of 5
4 of 5
5 of 5

Polyclonal Rabbit anti‑Human APEX1 / APE1 Antibody (IF, WB) LS‑C746818

Polyclonal Rabbit anti‑Human APEX1 / APE1 Antibody (IF, WB) LS‑C746818

Antibody:
APEX1 / APE1 Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$287
LS-C746818-20
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
APEX1 / APE1 Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
APE1 antibody LS-C746818 is an unconjugated rabbit polyclonal antibody to human APE1 (APEX1). Validated for IF, IP and WB.
Target
Human APEX1 / APE1
Synonyms
APEX1 | AP endonuclease class I | AP lyase | APE | APE-1 | APEN | APE1 | APEX nuclease | Redox factor-1 | AP endonuclease 1 | APEX | APX | Protein REF-1 | REF-1 | REF1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-318 of human APEX1 (NP_542380.1). MPKRGKKGAVAEDGDELRTEPEAKKSKTAAKKNDKEAAGEGPALYEDPPDQKTSPSGKPATLKICSWNVDGLRAWIKKKGLDWVKEEAPDILCLQETKCSENKLPAELQELPGLSHQYWSAPSDKEGYSGVGLLSRQCPLKVSYGIGDEEHDQEGRVIVAEFDSFVLVTAYVPNAGRGLVRLEYRQRWDEAFRKFLKGLASRKPLVLCGDLNVAHEEIDLRNPKGNKKNAGFTPQERQGFGELLQAVPLADSFRHLYPNTPYAYTFWTYMMNARSKNVGWRLDYFLLSHSLLPALCDSKIRSKALGSDHCPITLYLAL
Applications
  • Immunofluorescence (1:50 - 1:100)
  • Western blot (1:500 - 1:2000)
  • Immunoprecipitation (1:50 - 1:200)
Usage
The predicted MW is 35kDa, while the observed MW by Western blot was 39kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About APEX1 / APE1
P27695 NM_001641 NP_001632.2

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/3/2024