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Catalog Number	Size	Price
LS-C156592-400	400 µl	$393

APG4B / ATG4B Antibody - Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

APG4B / ATG4B Antibody - Fluorescent image of U251 cells stained with ATG4B antibody. U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated ATG4B primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ATG4B immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.

APG4B / ATG4B Antibody - Western blot of anti-APG4B antibody in HeLa cell lysate. APG4B (arrow) was detected using the purified antibody.

APG4B / ATG4B Antibody - Western blot of anti-hAPG4B-373 antibody in 293 cell line lysates transiently transfected with the ATG4B gene (2 ug/lane). hAPG4B-373(arrow) was detected using the purified antibody.

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Polyclonal Rabbit anti‑Human APG4B / ATG4B Antibody (aa358‑390, IHC, IF, WB) LS‑C156592

Name: Anti-APG4B / ATG4B Antibody | Rabbit anti-Human Polyclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C156592-400
Price: 393.0000 USD
Availability: InStock

Submit Review LSBio Guarantee

Antibody:

APG4B / ATG4B Rabbit anti-Human Polyclonal (aa358-390) Antibody

Application:

IHC, IHC-P, IF, WB

Reactivity:

Human

Format:

Unconjugated, Unmodified

$393

LS-C156592-400

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(800) 227-6666

For Research Use Only

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Overview

Antibody:

APG4B / ATG4B Rabbit anti-Human Polyclonal (aa358-390) Antibody

Application:

IHC, IHC-P, IF, WB

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

ATG4B antibody LS-C156592 is an unconjugated rabbit polyclonal antibody to human ATG4B (APG4B) (aa358-390). Validated for IF, IHC and WB.

Target

Human APG4B / ATG4B

Synonyms

See All APG4B / ATG4B Antibodies

Host

Rabbit

Reactivity

Human (tested or 100% immunogen sequence identity)

Predicted

Mouse (at least 90% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Ammonium sulfate precipitation

Modifications

Unmodified

Epitope

aa358-390

Specificity

This ATG4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 358-390 amino acids from the C-terminal region of human ATG4B.

Applications

IHC
IHC - Paraffin (1:50 - 1:100)
Immunofluorescence
Western blot (1:1000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

PBS, 0.09% Sodium Azide

Storage

Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About APG4B / ATG4B

Uniprot: Q9Y4P1 NCBI: NM_013325 NP_037457.3