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Antibodies > APP / Beta Amyloid Precursor > LS-C800945
 
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Catalog Number Size Price
LS-C800945-100 100 µl (1 mg/ml) $379 
LS-C800945-200 200 µl (1 mg/ml) $421 
APP / Beta Amyloid Precursor Antibody - 1:200 staining human kidney tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
APP / Beta Amyloid Precursor Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
APP / Beta Amyloid Precursor Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 min at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor® 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.
APP / Beta Amyloid Precursor Antibody - Western blot analysis of Amyloid beta A4 phosphorylation expression in HeLa whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
APP / Beta Amyloid Precursor Antibody - Western blot analysis of Phospho-Amyloid beta A4 (Thr743/668) expression in various lysates
APP / Beta Amyloid Precursor Antibody - 1:200 staining human kidney tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
APP / Beta Amyloid Precursor Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
APP / Beta Amyloid Precursor Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 min at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor® 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.
APP / Beta Amyloid Precursor Antibody - Western blot analysis of Amyloid beta A4 phosphorylation expression in HeLa whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
APP / Beta Amyloid Precursor Antibody - Western blot analysis of Phospho-Amyloid beta A4 (Thr743/668) expression in various lysates
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Polyclonal Rabbit anti‑Human APP / Beta Amyloid Precursor Antibody (phospho‑Thr743,phospho‑Thr668, IHC, IF, WB) LS‑C800945

Polyclonal Rabbit anti‑Human APP / Beta Amyloid Precursor Antibody (phospho‑Thr743,phospho‑Thr668, IHC, IF, WB) LS‑C800945

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Antibody:
APP / Beta Amyloid Precursor Rabbit anti-Human Polyclonal (pThr743,pThr668) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C800945-100
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
APP / Beta Amyloid Precursor Rabbit anti-Human Polyclonal (pThr743,pThr668) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Beta Amyloid Precursor antibody LS-C800945 is an unconjugated rabbit polyclonal antibody to Beta Amyloid Precursor (APP) (pThr743,pThr668) from human. It is reactive with human, mouse and rat. Validated for IF, IHC, Peptide-ELISA and WB.
Target
Human APP / Beta Amyloid Precursor
Synonyms
APP | ABPP | A4 | ABETA | AD1 | Alzheimer disease | Amyloid beta | Beta-amyloid peptide | APPI | Beta amyloid | CVAP | AAA | PN2 | PreA4 | Amyloid beta A4 protein | Peptidase nexin-II | CTFgamma | PN-II | Protease nexin-II
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human Amyloid beta A4 around the phosphorylation site of Threonine 743/668.
Epitope
pThr743,pThr668
Specificity
Phospho-Amyloid beta A4 (Thr743/668) Antibody detects endogenous levels of Amyloid beta A4 only when phosphorylated at Threonine 743/668.
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About APP / Beta Amyloid Precursor
P05067 NM_000484 NP_000475.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 5/14/2024
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