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Catalog Number Size Price
LS-C756518-10 10 µg $318 
LS-C756518-100 100 µg $470 
AREG / Amphiregulin Antibody - Western blot analysis of Amphiregulin using anti-Amphiregulin antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates,Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Amphiregulin antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Amphiregulin at approximately 41KD. The expected band size for Amphiregulin is at 28KD.

Polyclonal Rabbit anti‑Mouse AREG / Amphiregulin Antibody (IHC, WB) LS‑C756518

Polyclonal Rabbit anti‑Mouse AREG / Amphiregulin Antibody (IHC, WB) LS‑C756518

Antibody:
AREG / Amphiregulin Rabbit anti-Mouse Polyclonal Antibody
Application:
IHC-P, WB, ELISA
Reactivity:
Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C756518-10
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
AREG / Amphiregulin Rabbit anti-Mouse Polyclonal Antibody
Application:
IHC-P, WB, ELISA
Reactivity:
Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Amphiregulin antibody LS-C756518 is an unconjugated rabbit polyclonal antibody to Amphiregulin (AREG) from mouse. It is reactive with mouse and rat. Validated for ELISA, IHC and WB.
Target
Mouse AREG / Amphiregulin
Synonyms
AREG | Amphiregulin | CRDGF | SDGF
Host
Rabbit
Reactivity
Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E. coli-derived mouse Amphiregulin recombinant protein (Position:V100-K191).
Specificity
No cross reactivity with other proteins.
Applications
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Western blot (0.1 - 0.5 µg/ml)
  • ELISA (1 - 5 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
After reconstitution, may be stored at 4°C for 1 month. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About AREG / Amphiregulin
P15514 NM_001657 NP_001648.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/22/2024