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Antibodies > ARSA / Arylsulfatase A > LS-C756655
 
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Catalog Number Size Price
LS-C756655-10 10 µg $318 
LS-C756655-100 100 µg $470 
ARSA / Arylsulfatase A Antibody - IHC analysis of ARSA using anti-ARSA antibody. ARSA was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-ARSA Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ARSA / Arylsulfatase A Antibody - Western blot analysis of ARSA using anti-ARSA antibody. Electrophoresis was performed on a 10% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A375 whole cell lysate,Lane 2: human A549 whole cell lysate,Lane 3: human SMMC-7721 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-ARSA antigen affinity purified monoclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system.
ARSA / Arylsulfatase A Antibody - Western blot analysis of ARSA using anti-ARSA antibody. Electrophoresis was performed on a 10% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat testis tissue lysate,Lane 2: rat liver tissue lysate,Lane 3: rat brain tissue lysate,Lane 4: rat lung tissue lysate,Lane 5: mouse testis tissue lysate,Lane 6: mouse liver tissue lysate,Lane 7: mouse brain tissue lysate,Lane 8: mouse lung tissue lysate,Lane 9: mouse HEPA1-6 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-ARSA antigen affinity purified monoclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system.
ARSA / Arylsulfatase A Antibody - Flow Cytometry analysis of Raji cells using anti-ARSA antibody. Overlay histogram showing Raji cells stained with anti-ARSA antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ARSA Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
ARSA / Arylsulfatase A Antibody - Flow Cytometry analysis of ANA-1 cells using anti-ARSA antibody. Overlay histogram showing ANA-1 cells stained with anti-ARSA antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-ARSA Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
ARSA / Arylsulfatase A Antibody - IHC analysis of ARSA using anti-ARSA antibody. ARSA was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-ARSA Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ARSA / Arylsulfatase A Antibody - Western blot analysis of ARSA using anti-ARSA antibody. Electrophoresis was performed on a 10% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A375 whole cell lysate,Lane 2: human A549 whole cell lysate,Lane 3: human SMMC-7721 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-ARSA antigen affinity purified monoclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system.
ARSA / Arylsulfatase A Antibody - Western blot analysis of ARSA using anti-ARSA antibody. Electrophoresis was performed on a 10% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat testis tissue lysate,Lane 2: rat liver tissue lysate,Lane 3: rat brain tissue lysate,Lane 4: rat lung tissue lysate,Lane 5: mouse testis tissue lysate,Lane 6: mouse liver tissue lysate,Lane 7: mouse brain tissue lysate,Lane 8: mouse lung tissue lysate,Lane 9: mouse HEPA1-6 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-ARSA antigen affinity purified monoclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system.
ARSA / Arylsulfatase A Antibody - Flow Cytometry analysis of Raji cells using anti-ARSA antibody. Overlay histogram showing Raji cells stained with anti-ARSA antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ARSA Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
ARSA / Arylsulfatase A Antibody - Flow Cytometry analysis of ANA-1 cells using anti-ARSA antibody. Overlay histogram showing ANA-1 cells stained with anti-ARSA antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-ARSA Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Monoclonal Mouse anti‑Human ARSA / Arylsulfatase A Antibody (IHC, WB) LS‑C756655

Monoclonal Mouse anti‑Human ARSA / Arylsulfatase A Antibody (IHC, WB) LS‑C756655

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Antibody:
ARSA / Arylsulfatase A Mouse anti-Human Monoclonal Antibody
Application:
IHC-Fr, ICC, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C756655-10
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
ARSA / Arylsulfatase A Mouse anti-Human Monoclonal Antibody
Application:
IHC-Fr, ICC, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Arylsulfatase A antibody LS-C756655 is an unconjugated mouse monoclonal antibody to human Arylsulfatase A (ARSA). Validated for Flow, ICC, IHC and WB.
Target
Human ARSA / Arylsulfatase A
Synonyms
ARSA | Arylsulfatase A | Cerebroside-sulfatase | MLD | ASA
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the C-Terminus of human ARSA (454-482 aa QALKQLQLLKAQLDAAVTFGPSQVARGED), different from the related mouse sequence by six amino acids.
Specificity
No cross reactivity with other proteins.
Applications
  • IHC - Frozen (0.5 - 1 µg/ml)
  • ICC (0.5 - 1 µg/ml)
  • Western blot (0.1 - 0.5 µg/ml)
  • Flow Cytometry (1 - 3 µg/10E6 cells)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
After reconstitution, may be stored at 4°C for 1 month. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ARSA / Arylsulfatase A
P15289 NM_000487 NP_000478.3

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ARSA / Arylsulfatase A Antibody - Human Lung, Respiratory Epithelium: Formalin-Fixed, Paraffin-Embedded (FFPE)
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Applications: IHC, IHC - Paraffin, Western blot
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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
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Vector Laboratories, Inc.
6737 Mowry Ave
Newark, CA 94560
United States

Tel: (800) 227-6666 / (650) 697-3600

To place an order:
customerservice@vectorlabs.com

For questions about orders:
Customer.Support@LSBio.com

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