Target
Human ARTN / Artemin
Synonyms
ARTN | ENOVIN | EVN | Neurotrophic factor | Artemin | Neublastin
Reactivity
Human, Monkey, Bovine, Rabbit
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-NSTWRTVDRLS, from the internal region of the protein sequence according to NP_003967.2NP_476501.1NP_476431.2.
Specificity
Human ARTN / Artemin. This antibody is expected to recognize all reported isoforms (NP_003967.2; NP_476501.1; NP_476431.2). Reported variants represent identical protein: NP_476431.2, NP_001129687.1 Reported variants represent identical protein: NP_003967.2, NP_476432.2
Applications
- Western blot (0.1 - 0.3 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:8000)
Usage
Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: Approx 28kD band observed in lysates of cell lines HEK293, HepG2 and Kelly (calculated MW of 22.9kD according to NP_003967.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Pandey et al, Endocrinology. 2010 Mar;151(3):909-20; PMID: 20118197). Recommended concentration: 0.1-0.3 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee