Target
Human ATF1
Synonyms
ATF1 | ATF-1 | EWS-ATF1 | FUS/ATF-1 | Protein TREB36 | TREB36
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence EDSHKSTTSETAPQ-C, from the N-Terminus of protein sequence according to NP_005162.1.
Applications
- Western blot (0.1 - 0.3 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:8000)
Usage
Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: Approx 37kDa band observed in nuclear lysates of cell line A431 (calculated MW of 29.2kDa according to NP_005162.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.3 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee