Products
Research Areas
COVID-19
Resources
Login
Quick Order
Cart Cart lightblue
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Contact Us

Locations


Orders Processing,
Shipping & Receiving,
Warehouse

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464


Production Lab

Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036

Telephone Numbers



Tel: +1 (800) 227-6666


Contact Us



Additional Contact Details

Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-C808458-100 100 µl (1 mg/ml) $379 
LS-C808458-200 200 µl (1 mg/ml) $421 
ATF2 Antibody - 1:200 staining human kidney tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
ATF2 Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
ATF2 Antibody - Western blot analysis of extracts of rat brain tissue using Phospho-ATF2 (Ser62/44) antibody.
ATF2 Antibody - Western blot analysis of ATF2 phosphorylation expression in TNF-a treated HeLa whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
ATF2 Antibody - Western blot analysis of Phospho-ATF2 (Ser62 or 44) expression in various lysates
ATF2 Antibody - 1:200 staining human kidney tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
ATF2 Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
ATF2 Antibody - Western blot analysis of extracts of rat brain tissue using Phospho-ATF2 (Ser62/44) antibody.
ATF2 Antibody - Western blot analysis of ATF2 phosphorylation expression in TNF-a treated HeLa whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
ATF2 Antibody - Western blot analysis of Phospho-ATF2 (Ser62 or 44) expression in various lysates
1 of 5
2 of 5
3 of 5
4 of 5
5 of 5

Polyclonal Rabbit anti‑Human ATF2 Antibody (phospho‑Ser62,phospho‑Ser44, IHC, IF, WB) LS‑C808458

Polyclonal Rabbit anti‑Human ATF2 Antibody (phospho‑Ser62,phospho‑Ser44, IHC, IF, WB) LS‑C808458

Antibody:
ATF2 Rabbit anti-Human Polyclonal (pSer62,pSer44) Antibody
Application:
IHC, IF, WB, IP, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C808458-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
ATF2 Rabbit anti-Human Polyclonal (pSer62,pSer44) Antibody
Application:
IHC, IF, WB, IP, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
ATF2 antibody LS-C808458 is an unconjugated rabbit polyclonal antibody to ATF2 (pSer62,pSer44) from human. It is reactive with human, mouse and rat. Validated for IF, IHC, IP, Peptide-ELISA and WB.
Target
Human ATF2
Synonyms
ATF2 | CREB-2 | CRE-BP1 | CREB2 | HB16 | TREB7 | CREBP1
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human ATF2 around the phosphorylation site of Serine 62 or 44.
Epitope
pSer62,pSer44
Specificity
Phospho-ATF2 (Ser62 or 44) Antibody detects endogenous levels of ATF2 only when phosphorylated at Serine 62 or 44.
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Immunoprecipitation
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ATF2
P15336 NM_001880 NP_001871.2

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/3/2024