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Catalog Number Size Price
LS-C776035-100 100 µg (1 mg/ml) $479 
ATG3 Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG3 Polyclonal Antibody. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG3 Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ATG3 Antibody. (D) Composite.
ATG3 Antibody - Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~35.9 kDa ATG3 protein using Rabbit Anti-ATG3 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ATG3 Polyclonal Antibody  at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~35.9 kDa.
ATG3 Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG3 Polyclonal Antibody. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG3 Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ATG3 Antibody. (D) Composite.
ATG3 Antibody - Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~35.9 kDa ATG3 protein using Rabbit Anti-ATG3 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ATG3 Polyclonal Antibody  at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~35.9 kDa.
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Polyclonal Rabbit anti‑Human ATG3 Antibody (PerCP, IF, WB) LS‑C776035

Polyclonal Rabbit anti‑Human ATG3 Antibody (PerCP, IF, WB) LS‑C776035

Antibody:
ATG3 Rabbit anti-Human Polyclonal (PerCP) Antibody
Application:
IF, WB
Reactivity:
Human
Format:
Peridinin-chlorophyll-protein, Unmodified
Other formats:
Price
Catalog Number
$479
LS-C776035-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
ATG3 Rabbit anti-Human Polyclonal (PerCP) Antibody
Application:
IF, WB
Reactivity:
Human
Format:
Peridinin-chlorophyll-protein, Unmodified
Other formats:

Specifications

Description
ATG3 antibody LS-C776035 is a PerCP-conjugated rabbit polyclonal antibody to human ATG3. Validated for IF and WB.
Target
Human ATG3
Synonyms
ATG3 | APG3-LIKE | APG3 | APG3L | Autophagy related 3 | HApg3 | PC3-96 | Protein PC3-96 | Autophagy-related protein 3
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Peridinin-chlorophyll-protein. Also available conjugated with APC, Atto 390, Atto 488, Atto 594, Biotin, FITC, RPE.
Purification
Peptide Affinity Purified
Modifications
Unmodified
Immunogen
Synthetic peptide from the mid-protein of Human ATG3
Specificity
Detects ~35 kDa.
Applications
  • Immunofluorescence (1:100)
  • Western blot (1:1000)
  • Applications tested for the base form of this product only
Usage
Applications should be user optimized.
Presentation
PBS, 0.09% Sodium Azide, 50% Glycerol
Storage
Store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ATG3
Q9NT62 NM_022488 NP_071933.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/3/2024