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Catalog Number Size Price
LS-C776194-100 100 µg (1 mg/ml) $479 
ATG4D Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG4D Polyclonal Antibody. Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG4D Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ATG4D Antibody. (D) Composite.
ATG4D Antibody - Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of 52.9 kDa ATG4D protein using Rabbit Anti-ATG4D Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HeLa cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ATG4D Polyclonal Antibody  at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 52.9 kDa. Other Band(s): ~45 and 48 kDa.
ATG4D Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG4D Polyclonal Antibody. Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG4D Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ATG4D Antibody. (D) Composite.
ATG4D Antibody - Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of 52.9 kDa ATG4D protein using Rabbit Anti-ATG4D Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HeLa cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ATG4D Polyclonal Antibody  at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 52.9 kDa. Other Band(s): ~45 and 48 kDa.
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Polyclonal Rabbit anti‑Human ATG4D Antibody (Atto 594, IF, WB) LS‑C776194

Polyclonal Rabbit anti‑Human ATG4D Antibody (Atto 594, IF, WB) LS‑C776194

Antibody:
ATG4D Rabbit anti-Human Polyclonal (Atto 594) Antibody
Application:
IF, WB
Reactivity:
Human
Format:
Atto 594, Unmodified
Other formats:
Price
Catalog Number
$479
LS-C776194-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
ATG4D Rabbit anti-Human Polyclonal (Atto 594) Antibody
Application:
IF, WB
Reactivity:
Human
Format:
Atto 594, Unmodified
Other formats:

Specifications

Description
ATG4D antibody LS-C776194 is an Atto 594-conjugated rabbit polyclonal antibody to human ATG4D. Validated for IF and WB.
Target
Human ATG4D
Synonyms
ATG4D | APG4-D | APG4D | AUTL4 | APG4 autophagy 4 homolog D | Cysteine protease ATG4D | Autophagin-4
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Atto 594. Also available conjugated with APC, Atto 390, Atto 488, Biotin, FITC, PerCP, RPE.
Purification
Peptide Affinity Purified
Modifications
Unmodified
Immunogen
Synthetic peptide from the N-Terminus of Human ATG4D (aa. 98-108)
Specificity
Detects ~53 kDa.
Applications
  • Immunofluorescence (1:100)
  • Western blot (1:1000)
  • Applications tested for the base form of this product only
Usage
Applications should be user optimized.
Presentation
PBS, 0.09% Sodium Azide, 50% Glycerol
Storage
Store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ATG4D
Q86TL0 NM_032885 NP_116274.3

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/3/2024