Yeast ATG8 / Autophagy 8

Rabbit

Yeast (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.

Unmodified

Rabbit Anti-Apg8 antibody was prepared from rabbit serum after repeated immunizations with recombinant yeast Apg8 protein.

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.

• IHC
• Western blot (1:4000 - 1:8000)
• Immunoprecipitation
• ELISA (1:20000 - 1:100000)

This purified polyclonal antibody reacts with yeast APG8 by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. This antibody using the specified conditions may recognize other prominent intrinsic bands (UBLs or their conjugates). Other intrinsic bands are readily detectable in yeast lysates at lower antibody dilutions. A 14 kD band corresponding to yeast APG8 is detected. Most yeast cell lysates can be used as a positive control without induction or stimulation.

Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Reconstitute with 100 ul deionized water (or equivalent).

Store vial at 4°C prior to restoration. Product is stable at 4°C as an undiluted liquid for several weeks. For extended storage, aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.