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Catalog Number Size Price
LS-C407637-10 10 µg $318 
LS-C407637-100 100 µg $470 
ATXN3 / JOS Antibody - Ataxin 3 antibody IHC-paraffin. IHC(P): Human Lung Cancer Tissue.
ATXN3 / JOS Antibody - Ataxin 3 antibody Western blot. All lanes: Anti Ataxin 3 at 0.5 ug/ml. Lane 1: Rat Brain Tissue Lysate at 50 ug. Lane 2: COLO320 Whole Cell Lysate at 40 ug. Lane 3: HELA Whole Cell Lysate at 40 ug. Predicted band size: 42 kD. Observed band size: 42 kD.
ATXN3 / JOS Antibody - Flow Cytometry analysis of A549 cells using anti-ATXN3 antibody. Overlay histogram showing A549 cells stained with anti-ATXN3 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATXN3 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
ATXN3 / JOS Antibody - Ataxin 3 antibody IHC-paraffin. IHC(P): Human Lung Cancer Tissue.
ATXN3 / JOS Antibody - Ataxin 3 antibody Western blot. All lanes: Anti Ataxin 3 at 0.5 ug/ml. Lane 1: Rat Brain Tissue Lysate at 50 ug. Lane 2: COLO320 Whole Cell Lysate at 40 ug. Lane 3: HELA Whole Cell Lysate at 40 ug. Predicted band size: 42 kD. Observed band size: 42 kD.
ATXN3 / JOS Antibody - Flow Cytometry analysis of A549 cells using anti-ATXN3 antibody. Overlay histogram showing A549 cells stained with anti-ATXN3 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATXN3 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Polyclonal Rabbit anti‑Human ATXN3 / JOS Antibody (aa226‑254, IHC, WB) LS‑C407637

Polyclonal Rabbit anti‑Human ATXN3 / JOS Antibody (aa226‑254, IHC, WB) LS‑C407637

Antibody:
ATXN3 / JOS Rabbit anti-Human Polyclonal (aa226-254) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Rat, Dog
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C407637-10
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
ATXN3 / JOS Rabbit anti-Human Polyclonal (aa226-254) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Rat, Dog
Format:
Unconjugated, Unmodified

Specifications

Description
JOS antibody LS-C407637 is an unconjugated rabbit polyclonal antibody to JOS (ATXN3) (aa226-254) from human. It is reactive with human, rat and dog. Validated for IHC and WB.
Target
Human ATXN3 / JOS
Synonyms
ATXN3 | ATX3 | Ataxin 3 | Ataxin 3 variant h | AT3 | Ataxin 3 variant m | Ataxin 3 variant ref | Ataxin-3 | Olivopontocerebellar ataxia 3 | SCA3 | MJD1 | JOS | Josephin | MJD
Host
Rabbit
Reactivity
Human, Rat, Dog (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the C-Terminus of human Ataxin 3 (226-254 aa EEDLQRALALSRQEIDMEDEEADLRRAIQ), different from the related mouse and rat sequences by two amino acids.
Epitope
aa226-254
Specificity
Ubiquitous.
Applications
  • IHC
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Western blot (0.1 - 0.5 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
IHC: Antigen retrieval by boiling the paraffin sections in 10 mM citrate buffer, pH6.0, for 20 minutes is required for the staining of formalin/paraffin sections.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ATXN3 / JOS
P54252 NM_004993 NP_004984.2

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 11/3/2024