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Catalog Number	Size	Price
LS-C745289-25	25 µl (1 mg/ml)	$304

AURKB / Aurora-B Antibody - Western Blot shows detection of Aurora B protein at 39 kDa (predicted band size) in lane 1. Lane 1 : Extract from COS7 cells treated with Nocodazole (1ug/ml, 16 hrs). Lane 2 : Extract from COS7 cells treated with Nocodazole (1ug/ml, 16 hrs) incubated with the phosphopeptide immunogen. Primary Antibody : Aurora B (phospho T232) antibody diluted 1:500.

Polyclonal Rabbit anti‑Human AURKB / Aurora‑B Antibody (phospho‑Thr232, IF, WB) LS‑C745289

Name: Anti-AURKB / Aurora-B Antibody | Rabbit anti-Human Polyclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C745289-25
Price: 304.0000 USD
Availability: InStock

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Antibody:

AURKB / Aurora-B Rabbit anti-Human Polyclonal (pThr232) Antibody

Application:

IF, WB, ELISA

Reactivity:

Human, Mouse

Format:

Unconjugated, Unmodified

$304

LS-C745289-25

Toll Free North America

206-374-1102

For Research Use Only

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Overview

Antibody:

AURKB / Aurora-B Rabbit anti-Human Polyclonal (pThr232) Antibody

Application:

IF, WB, ELISA

Reactivity:

Human, Mouse

Format:

Unconjugated, Unmodified

Specifications

Description

Aurora-B antibody LS-C745289 is an unconjugated rabbit polyclonal antibody to Aurora-B (AURKB) (pThr232) from human. It is reactive with human and mouse. Validated for ELISA, IF and WB.

Target

Human AURKB / Aurora-B

Synonyms

See All AURKB / Aurora-B Antibodies

Host

Rabbit

Reactivity

Human, Mouse (tested or 100% immunogen sequence identity)

Clonality

IgG Polyclonal

Conjugations

Unconjugated

Purification

Affinity purified

Modifications

Unmodified

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region surrounding T232 of Human Aurora Kinase B protein.

Epitope

pThr232

Specificity

Reactivity occurs against human Aurora Kinase B pT232 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human Aurora Kinase B is minimal by ELISA. No reaction is expected against Aurora Kinase A. However, 100% sequence homology as indicated by BLAST analysis is on record for this protein from human, mouse, rat, cow, pig, dog and chimpanzee. Cross reactivity with Aurora Kinase B from other sources is not known.

Applications

Immunofluorescence
Western blot (1:250 - 1:2000)
ELISA (1:10000 - 1:50000)

Usage

Applications should be user optimized.

Presentation

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Storage

Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

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About AURKB / Aurora-B

Uniprot: Q96GD4 NCBI: NM_004217 NP_004208.2