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Catalog Number Size Price
LS-C97419-400 400 µl $393 
BAD Antibody - Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
BAD Antibody - Fluorescent confocal image of HeLa cells stained with phospho-Bad-S99 antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated phospho-Bad-S99 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the phospho-Bad-S99 mainly to the cytoplasm.
BAD Antibody - Dot blot of anti-hBad-pS99 Phospho-specific antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
BAD Antibody - Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
BAD Antibody - Fluorescent confocal image of HeLa cells stained with phospho-Bad-S99 antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated phospho-Bad-S99 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the phospho-Bad-S99 mainly to the cytoplasm.
BAD Antibody - Dot blot of anti-hBad-pS99 Phospho-specific antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
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Polyclonal Rabbit anti‑Human BAD Antibody (phospho‑Ser99, IHC, IF, WB) LS‑C97419

Polyclonal Rabbit anti‑Human BAD Antibody (phospho‑Ser99, IHC, IF, WB) LS‑C97419

Antibody:
BAD Rabbit anti-Human Polyclonal (pSer99) Antibody
Application:
IHC, IHC-P, IF, WB, DB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C97419-400
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
BAD Rabbit anti-Human Polyclonal (pSer99) Antibody
Application:
IHC, IHC-P, IF, WB, DB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
BAD antibody LS-C97419 is an unconjugated rabbit polyclonal antibody to human BAD (pSer99). Validated for DB, IF, IHC and WB.
Target
Human BAD
Synonyms
BAD | BBC2 | Bcl-2-like protein 8 | BCL2-binding component 6 | BCL2L8 | BBC6 | BCL-X/BCL-2 binding protein | Bcl-2-binding component 6 | Bcl2 antagonist of cell death | BCL2-binding protein | Bcl2-L-8
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Epitope
pSer99
Specificity
This Bad Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S99 of human Bad.
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Immunofluorescence (1:200)
  • Western blot (1:1000)
  • Dot Blot (1:500)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BAD
Q92934 NM_004322 NP_004313.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 11/28/2024