Human BDNF

Rabbit

Human, Mouse, Rat (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated. Also available conjugated with Biotin.

Immunoaffinity purified

Unmodified

E.coli derived recombinant Human BDNF.

Human BDNF

• Western blot (0.1 - 0.2 µg/ml)
• ELISA (0.5 - 2 µg/ml)

Immunohistochemistry: This antibody stained U-2 OS and U-251 MG cells. The primary antibody was incubated at 2 ug/ml overnight at 4°C followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary. Information and photo are courtesy of the Cell Profiling group, SciLifeLab Stockholm. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hBDNF (2 ug/ml), a concentration of 0.54-0.81 ug/ml of this antibody is required. ELISA: To detect hBDNF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human BDNF (LS-C104729) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hBDNF. Western Blot: To detect hBDNF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBDNF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Lyophilized PBS, pH 7.2

Reconstitute in sterile water to 0.1-1 mg/ml.

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P23560 NCBI: NM_001709 NP_001700.2