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Catalog Number Size Price
LS-C60102-100 100 µg (0.96 mg/ml) $567 
c-Met Antibody - Anti-c-Met pY1349pY1356 Antibody - Western Blot. Western blot of affinity purified anti-c-Met pY1349pY1356 antibody shows detection of phosphorylated c-Met. Human mammary epithelial cells (B5/589) were serum deprived and stimulated with (+) and without (-) HGF. Cell lysates were immunoprecipitated using a human anti-c-Met antibody, resolved by SDS-PAGE, transferred to PVDF membrane and probed using various antibodies. Lane 1 and 2 where probed using the anti-c-Met pY1349pY1356 antibody (#Anti-c-MET pY1349 pY1356 (RABBIT) Antibody), lane 3 and 4 where probed using an anti-phosphotyrosine antibody as phosphorylation control and lane 5 and 6 where probed using an anti-cMet antibody as a total Met loading control. Bands recognized at an apparent molecular weight of ~170 kD are total Met, ~145 kD phosphorylated Met beta chain in HGF +, ~150 kD phosphorylated Met in +/- HGF, ~50kD phosphorylated Met alpha chain. Personal communication with D. Bottaro and F. Cecchi, CCR-NCI, Bethesda, MD.
c-Met Antibody - Anti-c-Met pY1349pY1356 Antibody - Western Blot. Western blot of affinity purified anti-c-Met pY1349pY1356 antibody shows detection of phosphorylated c-Met. Human mammary B5/589 epithelial cells were serum-deprived and treated with or without HGF. Cell lysates were immunoprecipitated with the anti-c-Met antibody, resolved by SDS-PAGE, transferred to PVDF membrane, and probed with anti-c-Met pY1349pY1356. Personal communication, D. Bottaro and T. Ito, NCI, Bethesda, MD.
c-Met Antibody - Anti-c-Met pY1349pY1356 Antibody - Western Blot. Western blot of affinity purified anti-c-Met pY1349pY1356 antibody shows detection of phosphorylated c-Met. Human mammary epithelial cells (B5/589) were serum deprived and stimulated with (+) and without (-) HGF. Cell lysates were immunoprecipitated using a human anti-c-Met antibody, resolved by SDS-PAGE, transferred to PVDF membrane and probed using various antibodies. Lane 1 and 2 where probed using the anti-c-Met pY1349pY1356 antibody (#Anti-c-MET pY1349 pY1356 (RABBIT) Antibody), lane 3 and 4 where probed using an anti-phosphotyrosine antibody as phosphorylation control and lane 5 and 6 where probed using an anti-cMet antibody as a total Met loading control. Bands recognized at an apparent molecular weight of ~170 kD are total Met, ~145 kD phosphorylated Met beta chain in HGF +, ~150 kD phosphorylated Met in +/- HGF, ~50kD phosphorylated Met alpha chain. Personal communication with D. Bottaro and F. Cecchi, CCR-NCI, Bethesda, MD.
c-Met Antibody - Anti-c-Met pY1349pY1356 Antibody - Western Blot. Western blot of affinity purified anti-c-Met pY1349pY1356 antibody shows detection of phosphorylated c-Met. Human mammary B5/589 epithelial cells were serum-deprived and treated with or without HGF. Cell lysates were immunoprecipitated with the anti-c-Met antibody, resolved by SDS-PAGE, transferred to PVDF membrane, and probed with anti-c-Met pY1349pY1356. Personal communication, D. Bottaro and T. Ito, NCI, Bethesda, MD.
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Polyclonal Rabbit anti‑Human c‑Met Antibody (phospho‑Tyr1349/1356, WB) LS‑C60102

Polyclonal Rabbit anti‑Human c‑Met Antibody (phospho‑Tyr1349/1356, WB) LS‑C60102

Antibody:
c-Met Rabbit anti-Human Polyclonal (pTyr1349/1356) Antibody
Application:
WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$567
LS-C60102-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
c-Met Rabbit anti-Human Polyclonal (pTyr1349/1356) Antibody
Application:
WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
C-Met antibody LS-C60102 is an unconjugated rabbit polyclonal antibody to c-Met (pTyr1349/1356) from human. It is reactive with human, mouse and rat. Validated for ELISA and WB.
Target
Human c-Met
Synonyms
MET | AUTS9 | C-Met | HGF/SF receptor | HGFR | Met kinase | Proto-oncogene c-Met | Scatter factor receptor | SF receptor | HGF receptor | RCCP2 | Tyrosine-protein kinase Met
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity chromatography
Modifications
Unmodified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y1349 and Y1356 of human c-Met protein.
Epitope
pTyr1349/1356
Specificity
A BLAST analysis was used to suggest cross-reactivity with c-Met from human, mouse and rat based on 100% homology with the immunizing sequence. Cross-reactivity with c-Met from other sources has not been determined
Applications
  • Western blot (1 µg/ml)
  • ELISA (1:93000)
Usage
This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 150 kD in size corresponding to phosphorylated c-Met protein by western blotting in the appropriate cell lysate or extract. This phospho-specific polyclonal antibody reacts with human c-Met pY1349pY1356 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About c-Met
P08581 NM_000245 NP_000236.2

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Requested From: United States
Date Requested: 11/23/2024