Target
Human CALCR / Calcitonin Receptor
Synonyms
CALCR | Calcitonin receptor | CRT | CT-R | CTR | CT receptor
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG2a
Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Immunogen
Human CALCR (Accession # P79222).
Specificity
Recognizes human calcitonin receptor. Stains human CALCR transfectants but not irrelevant transfectants.
Applications
- Flow Cytometry (0.25 µg/ml)
Usage
Suitable for use in Flow cytometry: 0.25 ug/ml. Validated for flow cytometry using MCF-7 cells. Dilute this antibody to 25 ug/ml and add 10 ul of the diluted solution to 1-2.5x10^5 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled monoclonal antibodies may be visualized by adding a secondary developing reagent such as anti-mouse IgG conjugated to a fluorochrome. MCF-7 cells were stained with anti-CALCR (filled histogram) or isotype control (open histogram) followed by PE-conjugated anti-mouse antibody. Hybridoma. Mouse myeloma with B cells obtained from a mouse immunized with HEK293 cells transfected with human CALCR (Accession # P79222).
Presentation
Lyophilized from PBS, pH 7.4, 5% Trehalose
Reconstitution
Reconstitute in 200 ul sterile PBS
Storage
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CALCR / Calcitonin Receptor