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Catalog Number Size Price
LS-C801089-100 100 µl (1 mg/ml) $379 
LS-C801089-200 200 µl (1 mg/ml) $421 
CASR/Calcium Sensing Receptor Antibody - Staining LOVO cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
CASR/Calcium Sensing Receptor Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 min at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.
CASR/Calcium Sensing Receptor Antibody - Western blot analysis of Calcium Sensing Receptor phosphorylation expression in LOVO cells whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
CASR/Calcium Sensing Receptor Antibody - Western blot analysis of Phospho-Calcium Sensing Receptor (Thr888) expression in various lysates
CASR/Calcium Sensing Receptor Antibody - Staining LOVO cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
CASR/Calcium Sensing Receptor Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 min at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.
CASR/Calcium Sensing Receptor Antibody - Western blot analysis of Calcium Sensing Receptor phosphorylation expression in LOVO cells whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
CASR/Calcium Sensing Receptor Antibody - Western blot analysis of Phospho-Calcium Sensing Receptor (Thr888) expression in various lysates
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Polyclonal Rabbit anti‑Human CASR/Calcium Sensing Receptor Antibody (phospho‑Thr888, IF, WB) LS‑C801089

Polyclonal Rabbit anti‑Human CASR/Calcium Sensing Receptor Antibody (phospho‑Thr888, IF, WB) LS‑C801089

Antibody:
CASR/Calcium Sensing Receptor Rabbit anti-Human Polyclonal (pThr888) Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C801089-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
CASR/Calcium Sensing Receptor Rabbit anti-Human Polyclonal (pThr888) Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Calcium Sensing Receptor antibody LS-C801089 is an unconjugated rabbit polyclonal antibody to Calcium Sensing Receptor (CASR) (pThr888) from human. It is reactive with human, mouse and rat. Validated for IF, Peptide-ELISA and WB.
Target
Human CASR/Calcium Sensing Receptor
Synonyms
CASR | Ca sensing receptor | Ca2+ sensing receptor | Calcium-sensing receptor | FIH | EIG8 | GPRC2A | HHC | HHC1 | Hypocalciuric hypercalcemia 1 | PCAR1 | NSHPT | CAR | FHH
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human Calcium Sensing Receptor around the phosphorylation site of Threonine 888.
Epitope
pThr888
Specificity
Phospho-Calcium Sensing Receptor (Thr888) Antibody detects endogenous levels of Calcium Sensing Receptor only when phosphorylated at Threonine 888.
Applications
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CASR/Calcium Sensing Receptor
P41180 NM_000388 NP_000379.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/24/2024