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| Catalog Number | Size | Price |
|---|---|---|
| LS-C104786-50 | 50 µg | $356 |
| LS-C104786-100 | 100 µg | $396 |
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Polyclonal Rabbit anti‑Human CCL4 / MIP‑1 Beta Antibody (WB) LS‑C104786
Polyclonal Rabbit anti‑Human CCL4 / MIP‑1 Beta Antibody (WB) LS‑C104786
Note: This antibody replaces LS-C54214, LS-C42368, LS-C37259
Antibody:
CCL4 / MIP-1 Beta Rabbit anti-Human Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
CCL4 / MIP-1 Beta Rabbit anti-Human Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Specifications
Description
MIP-1 Beta antibody LS-C104786 is an unconjugated rabbit polyclonal antibody to human MIP-1 Beta (CCL4). Validated for ELISA and WB.
Target
Human CCL4 / MIP-1 Beta
Synonyms
CCL4 | ACT2 | Act-2 | AT744.1 | C-C motif chemokine 4 | Chemokine (C-C motif) ligand 4 | CC chemokine ligand 4 | HC21 | LAG1 | MIP1B | MIP1B1 | LAG-1 | SCYA2 | Secreted protein G-26 | SIS-gamma | MIP-1-beta | MIP-1-beta(1-69) | Small-inducible cytokine A4 | T-cell activation protein 2 | G-26 | Lymphocyte-activation gene 1 | PAT 744 | Protein H400 | SCYA4
Host
Rabbit
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated.
Also available conjugated with Biotin.
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
E.coli derived recombinant Human MIP-1beta.
Specificity
Human MIP-1beta
Applications
- Western blot (0.1 - 0.2 µg/ml)
- ELISA (0.5 - 2 µg/ml)
Usage
Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human pancreas adenocarcinoma. The recommended concentration is 1 mg/ml with 2 hour incubation at room temperature. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human MIP-1beta (50.00 ng/ml), a concentration of 1.75-3.00 ug/ml of this antibody is required. ELISA: To detect Human MIP-1beta by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human MIP-1beta (LS-C104795) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human MIP-1beta. Western Blot: To detect Human MIP-1beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human MIP-1beta is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Presentation
Lyophilized from PBS, pH 7.2
Reconstitution
Reconstitute with sterile water
Storage
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About CCL4 / MIP-1 Beta
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Featured Products
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Species:
Human, Mouse, Rat
Applications:
IHC, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human
Applications:
Western blot, ELISA
Reactivity:
Human
Range:
4.1-1000 pg/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/20/2024
Date Requested: 12/20/2024
