Human CCL5 / RANTES

Mouse

Human (tested or 100% immunogen sequence identity)

IgG2b Monoclonal

Unconjugated

Protein A purified

Unmodified

E.coli derived recombinant Human RANTES.

Human RANTES

• Western blot (0.5 - 1 µg/ml)
• ELISA (2 - 4 µg/ml)

Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human tonsil. The recommended concentration is 10 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6 citrate buffer is recommended. Optimal concentrations and conditions may vary. Protocol and staining provided by Dr. Lauren Binge, Laboratory of Prof. Charles Mackay, Monash Univeristy, Australia. *Additional Immunostaining data available. Please contact Tech Support for information. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human RANTES (100 ng/ml), a concentration of 3-5 ug/ml of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 ul/well), a concentration of 2-4 ug/ml of this antibody will detect at least 500 pg/ml of recombinant human RANTES when used with biotinylated antigen affinity purified anti-human RANTES (LS-C104720) as the detection antibody at a concentration of approximately 0.5-1 ug/ml. Western Blot: To detect hRANTES by Western Blot analysis this antibody can be used at a concentration of 0.5-1 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hRANTES is 0.25-2 ng/lane, under reducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions.

Lyophilized from PBS

Reconstitute with sterile water

Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P13501 NCBI: NM_002985 NP_002976.2