Human CCNE1 / Cyclin E1

Mouse

Human (tested or 100% immunogen sequence identity)

IgG Monoclonal

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Protein G purified

Unmodified

Recombinant human Cyclin E.

Recognizes human p52 cyclin E. Cross-reactivity with mouse and rat was not observed.

• ICC (10 µg/ml)
• Western blot (0.5 - 2 µg/ml)
• Immunoprecipitation
• ELISA

Suitable for use in ELISA, Western Blot, Immunoprecipitation, and Immunocytochemistry. Proteins were visualized by using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 4 ug immunoprecipitated cyclin E from 500 ug of human A431 RIPA cell lysate as confirmed by Western blot analysis of the immunoprecipitate using rabbit polyclonal anti-cyclin E. Immunocytochemistry: 10 ug/ml detected cyclin E in 50% ethanol/50% acetic acid-fixed human A431 cells.

0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P24864 NCBI: NM_001238 NP_001229.1