Target
Human CD274 / B7-H1 / PD-L1
Synonyms
CD274 | B7-H | B7 homolog 1 | B7-H1 | B7H1 | CD274 molecule | CD274 antigen | PDCD1 ligand 1 | PDL1 | PD-L1 | PDCD1L1 | PDCD1LG1 | Programmed death ligand 1
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k
Monoclonal
Conjugations
FITC.
Also available
Unconjugated or conjugated with
Biotin,
RPE.
Purification
Protein A purified
Immunogen
Recombinant human CD274(B7-H1).
Specificity
Recombinant CD274 and native cell surface CD274 expressed on stimulated PBMC.
Applications
- Flow Cytometry
- ELISA
- Applications tested for the base form of this product only
Usage
Ficoll prepared human peripheral blood mononuclear cells (PBMC) were stimulated overnight in culture with 5 ug/ml PHA. Five x 105 cells per tube were washed and pre incubated 5 minutes with 20^l of 250^g/ml human IgG (to block non specific binding), after which they were incubated 45 minutes on ice with 80 ^l of anti-human CD274/FITC at a 1:50 dilution (10 ^g/ml). They were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.5 log10 fluorescent units when compared to a Mouse IgG1/FITC negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 ^l of 0.5 mg/ml anti-CD274 antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 100 mM KCl, 0.04% Sodium Azide, 5% Glycerol, 0.2% BSA
Storage
Store at 4°C. Do not freeze. Protect from light.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CD274 / B7-H1 / PD-L1