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Catalog Number	Size	Price
LS-C343957-10	10 µg	$318
LS-C343957-100	100 µg (0.5 mg/ml)	$470

CD3E Antibody - CD3 epsilon antibody IHC-frozen: Rat Spleen Tissue.

CD3E Antibody - CD3 epsilon antibody IHC-frozen: Mouse Spleen Tissue.

CD3E Antibody - IHC analysis of CD3 Epsilon using anti-CD3 Epsilon antibody. CD3 Epsilon was detected in frozen section of rat spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-CD3 Epsilon Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

CD3E Antibody - CD3 epsilon antibody IHC-paraffin: Human Tonsil Tissue.

CD3E Antibody - CD3 epsilon antibody IHC-paraffin: Mouse Spleen Tissue.

CD3E Antibody - CD3 epsilon antibody IHC-paraffin: Rat Spleen Tissue.

CD3E Antibody - IF analysis of CD3E/CD68 using anti-CD3E/CD68 antibody CD3E/CD68 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/mL rabbit anti-CD3E Antibody/ mouse anti CD68 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG and Cy3 Conjugated Avidin were used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

CD3E Antibody - CD3 epsilon antibody Western blot. All lanes: Anti CD3 Epsilon at 0.5 ug/ml. Lane 1: JURKAT Whole Cell Lysate at 40 ug. Lane 2: CEM Whole Cell Lysate at 40 ug. Predicted band size: 23 kD. Observed band size: 23 kD.

CD3E Antibody - CD3 epsilon antibody Western blot. All lanes: Anti CD3 Epsilon at 0.5 ug/ml. WB : Recombinant Human CD3epsilon Protein 0.5ng . Predicted band size: 40 kD. Observed band size: 40 kD.

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Polyclonal Rabbit anti‑Human CD3E Antibody (aa23‑207, IHC, WB) LS‑C343957

Name: Anti-CD3E Antibody | Rabbit anti-Human Polyclonal ICC,IHC,WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-C343957-10
Price: 318.0000 USD
Availability: InStock
Rating: 4 (1 reviews)

(4.0) Rating

Submit Review (1) Publication(s) LSBio Guarantee

Note: This antibody replaces LS-C357768

Antibody:

CD3E Rabbit anti-Human Polyclonal (aa23-207) Antibody

Application:

IHC, IHC-P, IHC-Fr, ICC, WB

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

$318

LS-C343957-10

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

CD3E Rabbit anti-Human Polyclonal (aa23-207) Antibody

Application:

IHC, IHC-P, IHC-Fr, ICC, WB

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

Specifications

Description

CD3E antibody LS-C343957 is an unconjugated rabbit polyclonal antibody to CD3E (aa23-207) from human. It is reactive with human, mouse and rat. Validated for ICC, IHC and WB. Cited in 1 publication.

(1) Publication(s)

Target

Human CD3E

Synonyms

See All CD3E Antibodies

Host

Rabbit

Reactivity

Human, Mouse, Rat (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunogen affinity purified

Modifications

Unmodified

Immunogen

E.coli-derived human CD3 epsilon recombinant protein (Position: D23-I207). Human CD3 epsilon shares 65% amino acid (aa) sequence identity with mouse CD3 epsilon.

Epitope

aa23-207

Applications

IHC
IHC - Paraffin
IHC - Frozen
ICC
Western blot

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500µg/ml.

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About CD3E

NCBI: NM_000733 NP_000724.1

LSBio Ratings

CD3E Antibody (aa23-207) for IHC, ICC, WB/Western LS-C343957 has an LSBio Rating of

(4.0) Rating

Publications (4)

Learn more about The LSBio Ratings Algorithm

Publications (1)

Inhibition of STAT3 augments antitumor efficacy of anti-CTLA-4 treatment against prostate cancer. Kristina Witt , Susan Evans-Axelsson, Andreas Lundqvist, Martin Johansson, Anders Bjartell, Rebecka Hellsten. Cancer immunology, immunotherapy : CII. 2021 Nov;;70:3155-3166.

Full Text Article PubMed: 33786638 PMC: PMC8505385

Submit a Publication for LS-C343957