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Catalog Number	Size	Price
LS-C796669-100	100 µg	$539

CD5 Antibody - Immunohistochemical staining of paraffin-embedded mouse thymus tissue using anti-CD5 clone UMAB9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer. (pH8.5) at 120°C for 3 min. (1:300).

CD5 Antibody - Immunohistochemical staining of paraffin-embedded mouse spleen tissue using anti-CD5 clone UMAB9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer. (pH8.5) at 120 oC for 3 min. (1:300).

CD5 Antibody - Immunohistochemical staining of paraffin-embedded mouse ascending colon tissue using anti-CD5 clone UMAB9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer. (pH8.5) at 120°C for 3 min. (1:300).

CD5 Antibody - Immunohistochemical staining of paraffin-embedded human tonsil using anti-CD5 clone UMAB9 mouse monoclonal antibody at 1:200 dilution of 0.1mg/mL detection with Polink2 Broad HRP DAB requires heat-induced epitope retrieval with Citrate pH6.0 at 95-100C 20 minutes. The image shows strong membranous and cytoplasmic staining in >75 % of non germinal center cells of tonsil and <20% of the germinal center cells. No staining was seen in the squamous epithelia cells.

CD5 Antibody - Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CD5 mouse monoclonal antibody. (Clone UMAB9, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

CD5 Antibody - Immunofluorescent staining of Jurkat cells using CD5 mouse monoclonal antibody green). Actin filaments were labeled with TRITC-phalloidin. (red), and nuclear with DAPI. (blue). The three-color overlay image is located at the bottom-right corner.

CD5 Antibody - Western blot analysis of extracts. (35ug) from 2 cell lines lysates by using anti-CD5 monoclonal antibody. (1:500)

CD5 Antibody - Western Blot analysis of 10 different human tissue lysates. (10ug) by using anti-CD5 monoclonal antibody. (clone UMAB9, 1:500)

CD5 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY CD5. (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates. (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD5. (1:500)

CD5 Antibody - Flow cytometric Analysis of Jurkat cells, using anti-CD5 antibody. (Red), compared to a nonspecific negative control antibody. (Blue).

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Monoclonal Mouse anti‑Human CD5 Antibody (clone UMAB9, Carrier‑free, IHC, IF, WB) LS‑C796669

Name: Anti-CD5 Antibody | Mouse anti-Human Monoclonal Carrier-free | LSBio
Brand: Lifespan BioSciences
SKU: LS-C796669-100
Price: 539.0000 USD
Availability: InStock

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Antibody:

CD5 Mouse anti-Human Monoclonal (Carrier-free) (UMAB9) Antibody

Application:

IHC, IF, WB, Flo

Reactivity:

Human

Format:

Unconjugated, Carrier-free

Other formats:

Unmodified

$539

LS-C796669-100

Toll Free North America

(800) 227-6666

For Research Use Only

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Overview

Antibody:

CD5 Mouse anti-Human Monoclonal (Carrier-free) (UMAB9) Antibody

Application:

IHC, IF, WB, Flo

Reactivity:

Human

Format:

Unconjugated, Carrier-free

Other formats:

Unmodified

Specifications

Description

CD5 antibody LS-C796669 is an unconjugated mouse monoclonal antibody to human CD5. Validated for Flow, IF, IHC and WB.

Target

Human CD5

Synonyms

CD5 | CD5 antigen | CD5 antigen (p56-62) | CD5 molecule | Lymphocyte antigen T1/Leu-1 | LEU1 | T1

See All CD5 Antibodies

Host

Mouse

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

IgG1 Monoclonal

Clone

UMAB9

Conjugations

Unconjugated

Purification

Affinity chromatography

Modifications

Carrier-free. Also available Unmodified.

Immunogen

Full length human recombinant protein of human CD5 (NP_055022) produced in HEK293T cell.

Specificity

The specificity of this antibody has been validated by testing against a high-density Protein Microarray containing more than 17,000 recombinant proteins.

Applications

IHC (1:100)
Immunofluorescence (1:100)
Western blot (1:500)
Flow Cytometry (1:100)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

Applications should be user optimized.

Presentation

PBS, pH 7.3, 8% Trehalose

Reconstitution

Reconstitute with distilled water to 1 mg/ml. To use this carrier-free antibody for conjugation experiment, we strongly recommend you to perform another round of desalting process.

Storage

Store at -20°C. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About CD5

Uniprot: P06127 NCBI: NM_014207 NP_055022.2