Human CD63

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1,k Recombinant Monoclonal

rMX-49.129.5

Unconjugated

Protein A/G purified

Azide-free, Carrier-free. Also available Unmodified.

Smooth plasma membrane fraction of MeWo cells. Antigen Molecular Weight: 26 kDa (core protein) - 30-60 kDa (glycosylated)

This MAb recognizes protein of 26 kDa -60 kDa , which is identified as CD63. Its epitope is different from that of MAb LAMP3/529. The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.

• IHC - Paraffin (1 - 2 µg/ml)

Immunohistology (Formalin-fixed - 1-2 ug/ml for 30 minutes at RT - Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.) Optimal dilution for a specific application should be determined.

10 mM PBS

Store at -20°C to -80°C for up to 2 years.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P08962 NCBI: NM_001780 NP_001771.1