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Catalog Number	Size	Price
LS-C675301-50	50 µl	$316
LS-C675301-100	100 µl (0.25 mg/ml)	$386

CD9 Antibody - Immunohistochemistry Dilution at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.

CD9 Antibody - Immunofluorescence staining of MCF-7 cells diluted at 1:34,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

CD9 Antibody - Western Blot Positive WB detected in:U87 whole cell lysate All Lanes:CD9 antibody at 0.55µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 25 KDa Observed band size: 25 KDa

CD9 Antibody - Overlay histogram showing Jurkat cells stained with CD9 Antibody (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4?.The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4?. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.

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Monoclonal Rabbit anti‑Human CD9 Antibody (IHC, IF, WB) LS‑C675301

Name: Anti-CD9 Antibody | Rabbit anti-Human Monoclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C675301-50
Price: 316.0000 USD
Availability: InStock

Submit Review LSBio Guarantee

Antibody:

CD9 Rabbit anti-Human Monoclonal Antibody

Application:

IHC, IHC-P, IF, WB, Flo, ELISA

Reactivity:

Human

Format:

Unconjugated, Unmodified

$316

LS-C675301-50

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

CD9 Rabbit anti-Human Monoclonal Antibody

Application:

IHC, IHC-P, IF, WB, Flo, ELISA

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

CD9 antibody LS-C675301 is an unconjugated rabbit monoclonal antibody to human CD9. Validated for ELISA, Flow, IF, IHC and WB.

Target

Human CD9

Synonyms

See All CD9 Antibodies

Host

Rabbit

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

IgG Monoclonal

Conjugations

Unconjugated

Purification

Affinity chromatography

Modifications

Unmodified

Immunogen

A synthesized peptide

Applications

IHC
IHC - Paraffin
Immunofluorescence
Western blot
Flow Cytometry
ELISA

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

PBS, pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About CD9

Uniprot: P21926 NCBI: NM_001769 NP_001760.1

Publications (0)

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Featured Products

CD9 Antibody (clone SN4/C3‑3A2, Biotin) LS‑C35419

Species: Human

Applications: Flow Cytometry

$CD9 Antibody - Staining of normal human platelets with 0.25 ug of Biotin Mouse IgG1, K isotype control (open histogram) or 0.25\ug Biotin anti-human CD9 (SN4) (colored histogram) followed by PE-SAv. Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.$